Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing hydrocarbon
Reexamination Certificate
2011-04-12
2011-04-12
Weber, Jon P (Department: 1657)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing hydrocarbon
C435S257200, C435S410000, C435S431000
Reexamination Certificate
active
07923228
ABSTRACT:
Acceleration of botryococcenoids and growth by concomitant provision of appropriate light, minerals, and assimilable carbon. Specifically, methods, compositions and systems for the in vitro growth of hydrocarbons in photosynthetic organisms while maintaining a biologically exclusive monocultural environment, as for example, fromBotryococcusspecies, is disclosed. Niche-nutrients can include about 200 ppm to about 3% nitrogen, and about 100 ppm to about 15% P2O5, and about 100 ppm to about 3.5% K2O. In certain embodiments, the present invention relates to the growth of the Chlorophyta such asBotryococcussp. in a nutrient medium that includes up to 15% phosphates, at least 3 ppm soluble iron, and up to about 70 ppm soluble zinc. Also disclosed is a substantially pure culture ofBotryococcus brauniivar. Showa, strain Ninsei, having the ATCC Accession No. PTA-7441, its parts, and hydrocarbons produced therefrom.
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Gough Tiffany M
Nields, Lemack & Frame, LLC
Weber Jon P
LandOfFree
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