Methods and compositions for expression of transgenes in plants

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C800S298000, C435S320100

Reexamination Certificate

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10660097

ABSTRACT:
The invention provides isolated gamma coixin promoters useful for the expression of heterologous coding sequences in transgenic plants. Further provided by the invention are transgenic plants comprising such promoters, expression constructs for the introduction of heterologous coding sequences into plants, and methods of using the plants and nucleic acids provided by the invention.

REFERENCES:
patent: 6635806 (2003-10-01), Kriz et al.
patent: WO 95/06128 (1995-03-01), None
de Freitas Fa et al. Structural characterization and promoter activity analysis of the gamma-kafirin gene from sorghum. Mol Gen Genet. Oct. 28, 1994;245(2):177-86.
de Freitas Fa et al. GenBank Accession No. X62480, Feb. 13, 1995, S.vulgare gene for gamma-kafirin.
Belanger and Kriz, “Molecular basis for allelic polymorphism of the maize globulin-1 gene,”Genet., 129:863-872, 1991.
Chen et al., “Minimal regions in the Arabidopsis Pistillata promoter responsice to the apetala3/pistillata feedback control do not contain CarG box,”Sex Plant Reprod., 13:85-94, 2000.
U.S. Appl. No. 08/763,704, filed Dec. 9, 1996, issued as U.S. Patent Document 6,326,527.
de Freitas et al., “Structural characterization and promoter activity analysis of the gamma-kafirin gene from sorghum,”Molecular and General Genetics, 245(2):177-186, 1994.
Dehio and Schell, “Identification of plant genetic loci involved in a posttranscriptional mechanism for meiotically reversible transgene silencing,”Proc. Natl. Acad. Sci. USA, 91:5538-5542, 1994.
Donald et al., “Mutation of either G box or I box sequences profoundly affects expression from the Arabidopsis rbcS-1A promoter,”The EMBO Journal, 9(6):1717-1726, 1990.
Ingelbrecht et al., “Post-transcriptional silencing of reporter transgenes in tobacco correlates with DNA methylation,”Proc. Natl. Acad. Sci. USA, 91:10502-10506, 1994.
Jorgensen, “Altered gene expression in plants due to trans interactions between homologous genes,”Trends Biotechnol., 8:340-44, 1990.
Jorgensen, “Cosuppression, flower color patterns, and metastable gene expression states,”Science, 268:686-691, 1995.
Kim et al., “a 20 nucleotide upstream element is essential for the eopaline synthase (nos) promoter activity,”Plant Molecular Biology, 24:105-117, 1994.
Kriz et al., “Structural and transcriptional analysis of DNA sequences flanking genes that encode 19 kilodalton zeins,”Mol. Gen. Genet., 207(1):90-98, 1987.
Langridge and Feix, “A zein gene of maize is transcribed from two widely separated promoter regions,”Cell, 34:1015-1022, 1983.
Leite et al., “Nucleotide sequence of a cDNA Clone Encoding γ-coixin fromCoix lacryma-jobiseeds,”Plant Physiol. 97:1604-1605, 1991.
Leite et al., “Phylogenetic relationship of zeins and coixins as determined by immunological cross-reactivity and Southern Blot analysis,”Plant Mol. Biol. 14:743-751, 1990.
Lindbo et al., “Induction of a highly specific antiviral state in transgenic plants: implications for gene regulation and virus resistance,”Plant Cell, 5:1749-1759, 1993.
Matzke and Matzke, “How and why do plants inactivate homologous (trans)genes?,”Plant Physiol., 107:679-685, 1995.
Matzke et al., “A variety of epistatic interactions can occur between partially homologous transgene loci brought together by sexual crossing,”Mol. Gen. Genet., 236:379-86, 1993.
Matzke et al., “Homology-dependent gene silencing in transgenic plants: epistatic silencing loci contain multiple copies of methylated transgenes,”Mol. Gen. Genet., 244:219-229, 1994.
Meyer, “Understanding and controlling transgene expression,”Trends Biotechnol., 13:332-337, 1995.
Mueller et al., “Homology-dependent resistance transgenic virus resistance in plants related to homology-dependent gene silencing,”Plant J., 7:1001-1013, 1995.
Napoli et al., “Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genesin trans,” Plant Cell, 2:279-289, 1990.
Neto et al., “The involvement of opaque 2 on β-prolamin gene regulation in maize andCoixsuggests a more general role for this transcriptional activator,”Plant Mol. Biol. 27:1015-1029, 1995.
Neuhuber et al., “Susceptibility of transgene loci to homology-dependent gene silencing,”Mol. Gen. Genet., 244:230-241, 1994.
Ottoboni et al., “Sequence analysis of 22 KDA-like aplpha coixin genes reveals highly conserved protein structure and regulatory elements,”Plant Molecular Biology, 21(5):765-778, 1993.
Ottoboni et al., Sequence analysis of 22kDa-like α-coixin genes and their comparison with homologous zein and kafirin genes reveals highly conserved protein structure and regulatory elements,Plant Molecular Biology, 21:765-778, 1993.
Ottoboni et al., Sequence analysis of 22kDa-like α-coixin genes and their comparison with homologous zein and kafirin genes reveals highly conserved protein structure and regulatory elements, EMBL GenBank Database Accession No. X63113 from the World Wide Web at site: http://www.ncbi.nlm.nih.gov.
Park et al., “Gene silencing mediated by promoter homology occurs at the level of transcription and results in meiotically heritable alterations in methylation and gene activity,”Plant, 9:183-194, 1996.
Reina et al., “Sequence analysis of a genomic clone encoding a Zc2 protein from Zea mays W64 A,”Nucl. Acids. Res., 18(21):6426, 1990.
Siebert et al., “An improved PCR method of walking in uncloned genomic DNA,”Nucl. Acids Res., 23:1087-1088, 1995.
Van Blokland et al., “Transgene-mediated suppression of chalcone synthase expression inPetunia hybridaresults from an increase in RNA turnover,”Plant J., 6:861-877, 1994.
Van der Krol et al., “Flavonoid genes in petunia: addition of a limiting number of copies may lead to a suppression of gene expression,”Plant Cell, 2:291-99, 1990.
Vaucheret, “Identification of a general silencer for 19S and 35S promoters in a transgenic tobacco plant: 90bp of homology in the promoter sequence are sufficient for trans-inactivation,”C.R. Acad. Sci. III, 316:1471-83, 1993.
Vettore et al., “The molecular and functional characterization of an Opaque2 homologue gene from Coix and a new classification of plant bZIP proteins,”Plant Molecular Biology, 36(2):249-263, 1998.
Vettore et al., “The molecular and functional characterization of anOpaque2homologue gene fromCoixand a new classification of plant bZIP proteins,”Plant Mol. Biol. 36:249-263, 1998.
Wandelt and Feix, “Sequence of a 21 kd zein gene from maize containing an in-frame stop codon,”Nucl. Acids Res., 17(6):2354, 1989.
Yunes et al., “The transcriptional activator Opaque2 recognizes two different target sequences in the 22-kD-like α-prolamin genes,”The Plant Cell6:237-249, Feb. 1994.

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