Methods and compositions for enhanced plant cell transformation

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

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C435S468000, C435S418000, C435S419000, C800S278000

Reexamination Certificate

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10664658

ABSTRACT:
Methods and compositions to increaseAgrobacteriumtransformation efficiency (frequencies) in both dicot and monocot host plants include adding histones to the host plant at most transiently, and using histones and L-cysteine at certain stages in monocot transformation.

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Jefferson, R.A., et al. (1987) “GUS Fusions: β-Glucuronidase as a Sensitive and Versatile Gene Fusion Marker in Higher Plants.”EMBO J6(13):3901-3907.
Koncz, c. and Schell, J. (1986) “The Promoter of TL-DNA Gene 5 Controls the Tissue-Specific Expression of Chimeric Genes Carried by a Novel Type of Agrobacterium Binary Vector.”Mol Gen Genet204:383-396.
Lichtenstein, G., and Draper, J. (1986) “Genetic of Engineering Plants.”In Glover, D.M.(ed.)DNA Cloning: A Practical Approach2:67-119 (IRL Press, Oxford).
Matsumoto, S., et al. (1990)- “Integration ofAgrobacteriumT-DNA into a Tobacco Chromosome: Possible Involvement of DNA Homology between T-DNA and Plant DNA.”Mol Gen Genet224:309-316.
Mysore, K.S. et al. (1998) “Role of theAgrobacterium TumefaciensVirD2 Protein in T-DNA Transfer and Integration.”American Phytopathological Society11(7):668-683.
Narasimhulu, S.B., et al. (1996). “Early Transcription of Agrobacterium T-DNA Genes in Tobacco and Maize.”Plant Cell8:873-886.
NI, M., et al.(1995) “Strength and Tissue Specificity of Chimeric Promoters Derived from the Octopine and Mannopine Synthase Genes.”Plant J7(4):661-676.
Ohba, T., et al.(1995) “DNA Rearrangement Associated with the Integration of T-DNA in Tobacco: An Example for Multiple Duplications of DNA Around the Integration Target.”Plant J7(1):157-164.
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Zupan, J. Zam

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