Methods and compositions for effecting homologous recombination

Details Methods and compositions for effecting homologous recombination Methods and compositions for effecting homologous recombination

2005-08-30

2005-08-30

Fredman, Jeffrey (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S440000, C435S462000, C435S463000, C435S091200, C536S023100

Reexamination Certificate

active

06936418

ABSTRACT:
This invention concerns a method for effecting directed mutagenesis based on a guided homologous recombination system that comprises (i) a triple helix forming oligonucleotide, (ii) a donor nucleic acid segment, and (iii) an adapter segment comprising an oligonucleotide sequence able to bind at least a portion of said donor nucleic acid through Watson-Crick base pairing.

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