Methods and compositions for effecting homologous recombination

Details Methods and compositions for effecting homologous recombination Methods and compositions for effecting homologous recombination

1995-06-07

1998-07-07

Jones, W. Gary

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 5, 435 6, 435 911, 435 912, C12N 1563, C12Q 170, C12Q 168, C12P 1934

Patent

active

057767440

ABSTRACT:
Methods, compositions and kits for effecting homologous recombination are described. In one aspect, the method utilizes two introduced DNAs: (1) a mutagen-linked single-stranded oligonucleotide capable of specifically binding to double-stranded DNA to form a triple-stranded helix, and (2) a donor DNA fragment capable of undergoing homologous recombination with DNA targeted by the oligonucleotide.

REFERENCES:
Kim et al, "Probing the structure of a putative intermediate in homologous recombination: The third strand in the parallel DNA triplex is in contact with the major groove of the duplex", J. Mol. Biol. 247:874-889, 1995.
Camerini-Otero et al, "Parallel DNA triplexes, homologous recombination and other homology dependent DNA interactions", Cell 73:217-223, Apr. 1993.
Rao et al, "Homologous recognition and triplex formation promoted by RecA protein between duplex oligonucleotides and single-stranded DNA", J. Mol. Biol. 229:228-343, 1993.
Sandor et al, "Triple helix directed psoralen adducts induce a low frequency of recombination in an SV40 shuttle vector", Biochimica et Biophysica Acta 1263:235-240, Sep. 1995.
Wang et al, "Targeted mutagenesis in mammalian cells mediated by intracellular triple helix formation", Mol. Cell. Biol. 15(3): 1759-1768, Mar. 1995.
Rooney et al, "Antiparallel, intramolecular triplex DNA stimulates homologous recombination in cells", Proc. Natl. Acad. Sci. 92:2141-2144, Mar. 1995.
Kim et al, "Parallel DNA triplexes and homologous recombination", in Structural Biology: The State of the Art, Proceedings of the Eighth Conversation, Adenine Press, Albany, New York, pp. 67-74, 1994.

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