Methods and compositions for DNA mediated gene silencing

Details Methods and compositions for DNA mediated gene silencing Methods and compositions for DNA mediated gene silencing

2007-11-13

2007-11-13

Whiteman, Brian (Department: 1635)

Chemistry: molecular biology and microbiology

Vector, per se

Reexamination Certificate

active

10202479

ABSTRACT:
DNA compositions that mediate gene silencing via RNA interference are provided. Also provided are methods of using such compositions to silence gene expression in a cell.

REFERENCES:
patent: 5624803 (1997-04-01), Noonberg et al.
patent: 5733762 (1998-03-01), Midoux et al.
patent: 5908777 (1999-06-01), Lee et al.
patent: 6423885 (2002-07-01), Waterhouse et al.
patent: 6506559 (2003-01-01), Fire et al.
patent: 2002/0086356 (2002-07-01), Tuschl et al.
patent: 2003/0027168 (2003-02-01), Gan et al.
patent: WO96/26963 (1996-09-01), None
patent: WO99/32619 (1999-07-01), None
patent: WO 99/53050 (1999-10-01), None
patent: WO 01/12824 (2001-02-01), None
patent: WO01/29058 (2001-04-01), None
patent: WO01/42443 (2001-06-01), None
patent: WO01/68836 (2001-09-01), None
patent: WO01/75164 (2001-10-01), None
patent: WO01/92513 (2001-12-01), None
patent: WO01/96584 (2001-12-01), None
patent: WO02/44321 (2002-06-01), None
patent: WO 02/059257 (2002-08-01), None
patent: WO 02/059294 (2002-08-01), None
patent: WO 03/046186 (2003-06-01), None
Elbashir et al. (Nature vol. 411, May 24, 2001 494-498.
Lee et al., “Expression of small interfering RNAs targeted against HIV-1revtranscripts in human cells,”Nature Biology,vol. 19, May 2002, pp. 500-505.
Yu et al., “RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells,”PNAS,vol. 99, No. 9, Apr. 30, 2002, pp. 6047-6052.
Sui et al., “A DNA vector-based RNAi technology to suppress gene expression in mammalian cells,” PNAS, vol. 99, No. 8, Apr. 16, 2002, pp. 5515-5520.
Brummelkamp et al., “A System for Stable Expression of Short Interfering RNAs in Mammalian Cells,”Science,vol. 296, Apr. 19, 2002, pp. 550-553.
Paddison et al., “Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells,”Genes&Development,vol. 16, Mar. 8, 2002, pp. 948-958.
Caplen et al., “Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems,”PNAS,vol. 98, Aug. 14, 2001, pp. 9742-9747.
Elbashir et al., “Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells,”Nature,vol. 411, May 24, 2001, pp. 494-498.
Parrish et al., “Functional Anatomy of a dsRNA Trigger: Differential Requirement for the Two Trigger Strands in RNA Interference,”Molecular Cell,vol. 6, Nov. 2000, pp. 1077-1087.
Kennerdell and Carthew, “Use of dsRNA-Mediated Genetic Interference to Demonstrate thatfrizzledandfrizzled 2Act in the Wingless Pathway,”Cell,vol. 95, Dec. 23, 1998, pp. 1017-1026.
Fire et al., “Potent and specific genetic interference by double-stranded RNA inCaenorhabditis elegans,” Nature,vol. 391, Feb. 19, 1998, pp. 806-811.
Stünkel et al., “A Nucleosome Positioned in the Distal Promoter Region Activates Transcription of the Human U6 Gene,”Molecular and Cellular Biology,17:8, Aug. 1997, pp. 4397-4405.
Lin, S-L et al., “D-RNAi (Messenger RNA-antisense DNA Interference) as a Novel Defense System Against Cancer and Viral Infections”,Bentham Science,vol. 1, No. 3, pp. 241-247, 2001.
Miyagishi, M. et al, “U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targetd gene expresion in mammalian cells”,Nature Biotechnology,vol. 19, No. 5, pp. 497-500, 2002.
Sui, G. et al., “A DNA vector-based RNAi technology to suppress gene expression in mammmalian cells”,PNAS,vol. 99, No. 8, pp. 5515-5520, 2002.

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