Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1994-07-29
1997-09-23
Knode, Marian C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435820, 530326, C12Q 170, G01N 33576, C07K 708
Patent
active
056703104
ABSTRACT:
The present invention provides antigenic peptides which bind anti-HCV antibodies for the differential diagnosis of acute and chronic HCV infection. The invention further provides a method of differentiating acute and chronic hepatitis C virus infection in a subject comprising: a) contacting an antibody-containing sample from the subject with one or more of the peptides selected from the group consisting of peptide 59, comprising the amino acids AFASRGNHVSPTHYVPESDA (SEQ ID NO:1), peptide 137, comprising the amino acids MNRLIAFASRGNHVSPTHYV (SEQ ID NO:2) and peptide 138, comprising the amino acids SRGNHVSPTHYVPESDAAAR (SEQ ID NO:3) under conditions that permit binding between the peptide and the antibodies; b) detecting the presence of binding between the peptide and the antibodies; c) contacting the antibody-containing sample from the subject with an amount of peptide 139, comprising the amino acids NHVSPTHYVPESDAAARVTA (SEQ ID NO:4) under conditions that permit binding between the peptide and the antibodies; d) detecting the presence of binding between the peptide and the antibodies; and comparing the strength of the antibody binding of step b) with the strength of the antibody binding of step d), a stronger binding in step b) as compared to the binding in step d) indicating acute hepatitis C virus infection and an equivalent binding in both steps b) and d) indicating chronic hepatitis C virus infection. The present invention further provides a method of diagnosing a hepatitis C virus infection in a subject comprising contacting an antibody containing sample from the subject with a peptide comprising the amino acids SPTHYV (SEQ ID NO:5) and determining the presence of binding between the peptide and the antibodies from the sample, the presence of binding between the peptide and the antibodies indicating a hepatitis C virus infection.
REFERENCES:
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Wong, et al., "Longitudinal Analysis of the Humoral Immune Response to Human Immunodeficiency Virus Type 1 (HIV-1) gp160 Epitopes in Rapidly Progressing and Nonprogressing HIV-1-Infected Subjects," The Journal of Infectious Diseases 1993 168:1523-1527 (Dec. 1993).
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Fields Howard A.
Khudyakov Yury E.
Knode Marian C.
The United States of America as represented by the Department of
Wortman Donna C.
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