Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-10-25
1999-10-05
Myers, Carla J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 243, 536 2431, 536 2432, 435 5, C12Q 168, C07H 2104
Patent
active
059622253
ABSTRACT:
Methods and compositions are provided for the detection of specific nucleic acid sequences purified from cellular or tissue sources. More particularly, the present invention includes methods and compositions for the detection of nucleic acid sequences using a protection molecule that forms a protected nucleic acid sequence (PNAS) such as a triplex or duplex nucleic acid structure that includes the target nucleic acid sequence. An assay using the methods of the present invention may include one, two or three levels of specificity to minimize false positive signals. An assay using the methods or compositions of the present invention can be performed on large amounts of purified DNA in a single test, with high levels of sensitivity, thus eliminating the need for DNA amplification procedures.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4775619 (1988-10-01), Urdea
patent: 5176996 (1993-01-01), Hogan et al.
patent: 5273881 (1993-12-01), Sena et al.
patent: 5482836 (1996-01-01), Cantor et al.
patent: 5518901 (1996-05-01), Murtagh
patent: 5639609 (1997-06-01), Kruse-Muellar et al.
Berger et al. editors Guide to Molecular Cloning Techniques Methods in Enzymology 152:612-613 & 625-626, 1987.
Favolaro, et al., "Transcription Maps of Polyoma Virus-Specific RNA: Analysis by Two-Dimensional Nuclease S1 Gel Mapping", Methods in Enzymology, vol. 65, pp. 718-749.
Cassiday et al., "Replica Plating of Colonies from Listeria-Agars to Blood Agar to Improve the Isolation of Listeria monocytogenes from Foods" Applied and Environmental Microbiology, vol. 56, No. 7, Jul., pp. 2274-2275 (1990).
Chambers, Henry "Coagulase-Negative Staphylococci Resistant to .beta.-Lactam Antibiotics in vivo Produce Penicillin-Binding Protein 2a", Antimicrobial Agents and Chemotherapy, vol. 31, No. 12, pp. 1919-1924 (Dec. 1987).
Landegren et al., "DNA Diagnostics-Molecular Techniques and Automation", Science, vol. 242, pp. 229-237 (Oct. 14, 1988).
Blume et al., "Triple helix formation by purine-rich oligonucleotides targeted to the human dihydrofolate reductase promotor", Nucleic Acids Research, vol. 20, No. 7, pp. 1777-1784.
Lee et al., "Poly(pyrimidine).cndot.poly(purine) synthetic DNAs containing 5-methylcytosine form stable triplexes at neutral pH", Nucleic Acids Research, vol. 12, No. 16, pp. 6603-6614 (1984).
Aviv et al., "Purification of Biologically Active Globin Messenger RNA by Chromatograply on Oligothymidylic acid-Cellulose", Proc. Nat. Acad. Sci., vol. 69, No. 6, pp. 1408-1412 (Jun. 1972).
Urdea et al., "A novel method for the rapid detection of specific nucleotide sequences in crude biological samples without blotting or radioactivity; application to the analysis of hepatitis B virus in human serum", Gene, 61 pp. 253-264 (1987).
Guatelli et al., "Isothermal in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication", Proc. Natl. Acad. Sci., vol. 87, pp. 1874-1878 (Mar., 1990).
Kwoh et al., "Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format", Proc. Natl. Acad. Sci., vol. 86, pp. 1173-1177 (Feb. 1989).
Summers, William C., "A Simple Method for Extraction of RNA from E. coli Utilizing Diethyl Pyrocarbonate", Analytical Biochemistry, "Short Communications", 33(2), pp. 459-463 (1970).
Chirgwin et al., "Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease", Biochemistry, vol. 18, No. 24, pp. 5294-5299 (1979).
Dhingra et al., "Hybridization Protection Assay: A Rapid, Sensitive, and Specific Method for Detection of Philadelphia Chromosome-Positive Leukemias", Blood, vol. 77, No. 2, pp. 238-242 (Jan. 15, 1991).
Goodwin, Graham H., "The analysis of sequence-specific DNA-binding proteins in cell extracts", Gel Electrophoresis of Nucleic Acids--A Practical Approach, Second Edition, Oxford University Press, pp. 225-247 (1990).
Lee, et al., "Isolation of DNA from Fungal Mycelia and Single Spores", PCR Protocols: A Guide to Methods and Applications, Academic Press, Inc., pp. 282-287 (1990).
Kolberg, et al., "The Specificity of pilin DNA sequences for the detection of pathogenic Neisseria", Molecular and Cellular Probes, vol. 3, pp. 59-72 (1989).
Reddy, et al., "Bacterial RNA Isolation with One Hour Centrifugation in a Table-Top Ultracentrifuge", BioTechniques, vol. 8, No. 3, pp. 250-251 (1990).
Chomczynski, et al., "Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction", Analytical Biochemistry, vol. 162, pp. 156-159 (1987).
Palmiter, Richard D., "Magnesium Precipitation of Ribonucleoprotein Complexes. Expedient Techniques for the Isolation of Undegraded Polysomes and Messenger Ribonucleic Acid", Biochemistry, vol. 13, No. 17, pp. 3606-3615 (1974).
Wu, et al., "The Ligation Amplification Reaction (LAR-Amplification of Specific DNA Sequences Using Sequential Rounds of Template-Dependent Ligation" Genomics, vol. 4, pp. 560-569 (1989).
Actor et al. "A Bioluminescent Assay Using AquaLite for RT-PCR Amplified RNA from Mouse Lung", The Journal of NIH Research, vol. 8, p. 62 (Oct. 1996).
Lewis, Ricky, "GEN's 10 Best Biotechnology Research Areas", Genetic Engineering News, vol. 10:1, p. 24 (1990).
Lizardi et al., "Exponential Amplification of Recombinant-RNA Hybridization Probes", Bio/Technology, vol. 6, pp. 1197-1202 (Oct. 1988).
Cygene, Inc.
Myers Carla J.
LandOfFree
Methods and compositions for detection of specific nucleotide se does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods and compositions for detection of specific nucleotide se, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for detection of specific nucleotide se will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1170024