Methods and compositions for detection of a target nucleic...

Details Methods and compositions for detection of a target nucleic... Methods and compositions for detection of a target nucleic...

2007-02-01

2009-12-01

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C536S022100, C536S023100, C536S024300

Reexamination Certificate

active

07625705

ABSTRACT:
The invention provides compositions, kits and methods of generating a signal indicative of the presence of a target nucleic acid sequence in a sample by forming a cleavage structure. The cleavage structure is formed by incubating a sample containing a target nucleic acid with a downstream probe that forms a 3′ flap when hybridized to the target. The cleavage structure is cleaved with a 3′ nuclease and a detectable signal is produced.

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Ugozzoli et al. Detection of specific alleles by using allele -specific primer extension followed by capture on solid support. GATA 9(4) :107-112 (1992).

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