Methods and compositions for detection and analysis of...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C536S023100, C536S026600

Reexamination Certificate

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10600286

ABSTRACT:
Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor PNA complementary to the target polynucleotide. The sensor PNA comprises a signaling chromophore to absorb energy from the excited multichromophore and emit light in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.

REFERENCES:
patent: 4948843 (1990-08-01), Roberts et al.
patent: 4950587 (1990-08-01), Roberts et al.
patent: 5408109 (1995-04-01), Heeger et al.
patent: 5612221 (1997-03-01), Simons et al.
patent: 5869350 (1999-02-01), Heeger et al.
patent: 5881083 (1999-03-01), Diaz-Garcia et al.
patent: 5968762 (1999-10-01), Jadamec et al.
patent: 5990479 (1999-11-01), Weiss et al.
patent: 6280933 (2001-08-01), Glazer et al.
patent: 6534329 (2003-03-01), Heeger et al.
patent: 6743640 (2004-06-01), Whitten
patent: 2002/0009728 (2002-01-01), Bittner
patent: 2002/0034747 (2002-03-01), Bruchez
patent: 2002/0150759 (2002-10-01), Jones
patent: 2002/0177136 (2002-11-01), McBranch
patent: 2003/0054413 (2003-03-01), Kumaraswamy
patent: 2004/0241768 (2004-12-01), Whitten
patent: WO 99/35288 (1999-07-01), None
patent: WO 00/14278 (2000-03-01), None
patent: WO 00/66790 (2000-11-01), None
patent: WO 02/081735 (2002-10-01), None
patent: WO 2004/001379 (2003-12-01), None
Weiler et al. “Hybridization based DNA screening on peptide nucleic acid (PNA) oligomer arrays” Nucleic Acids Research, 1997, vol. 25, No. 14, pp. 2792-2799.
U.S. Appl. No. 60/202,647, filed May 8, 2000, Whitten.
U.S. Appl. No. 60/226,902, filed Aug. 23, 2000, Whitten.
U.S. Appl. No. 60/230,186, filed Sep. 1, 2000, Phillips.
U.S. Appl. No. 60/237,000, filed Sep. 29, 2000, Bruchez.
U.S. Appl. No. 60/240,216, filed Oct. 13, 2000, Bruchez.
U.S. Appl. No. 60/276,090, filed Mar. 16, 2001, Jones.
U.S. Appl. No. 60/314,094, filed Aug. 23, 2001, Burdick.
U.S. Appl. No. 60/314,101, filed Aug. 23, 2001, Whitten.
Wang et al., “Size-Specific Interactions Between Single- and Double-Stranded Oligonucleotides and Cationic Water-Soluble Oligofluorenes”, Adv. Funct. Mater., Jun. 2003, 13(6), 463-467.
Stork et al., “Energy Transfer in Mixtures of Water-Soluble Oligomers: Effect of Charge, Aggregation, and Surfactant Complexation”, Adv. Mater., Mar. 2002, 14(5), 361-366.
Lecler{grave over (c)}, “Optical and Electrochemical Transducers Based on Fucntionalized Conjugated Polymers”, Adv. Mater., 1999, 11(18), 1491-1498.
Balakin et al., “Conjugates of oligonucleotides with polyaromatic fluorophores as promising DNA probes”, Biosensors & Bioelectronics, 1998, 13, 771-778.
Ho et al., “Colorimetric and Fluormetric Detection of Nucleic Acids Using Cationic Polythiophene Derivatives”, Angew. Chem. Int. Ed., 2002, 41(9), 1548-1551.
McQuade et al., “Conjugated Polymer-Based Chemical Sensors”, Chem. Rev., 2000, 100, 2537-2574.
Chen et al., “Highly sensitive biological and chemical sensors based on reversible fluorescence quenching in a conjugated polymer”, PNAS, Oct. 1999, 96(22), 12287-12292.
Liu et al., “Effect of Chromophore-Charge Distance in the Energy Transfer Properties of Water-Soluble Conjugated Oligomers”, J. Am. Chem. Soc., 2003, 125, 6705-6714.
Gaylord et al., “DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes”, PNAS, Aug. 2002, 99(17), 10954-10957.
Bronich et al., “Recognition of DNA Topology in Reactions between Plasmids DNA and Cationic Copolymers”, J. Am. Chem. Soc., Sep. 2000, 122(35), 8339-8343.
Chen et al., “Tuning the Properties of Conjugated Polyelectrolytes through Surfactant Complexation”, J. Am. Chem. Soc., 2000, 122, 9302-9303.
Gaylord et al., “Water-Soluble Conjugated Oligomers: Effect of Chain Length and Aggregation on Photoluminescene-Quenching Efficiencies”, J. Am. Chem. Soc., 2001, 123, 6417-6418.
Hong et al., “Water-Soluble Oligmer Dimers Based on Para cyclophane: A New optical Platform for Fluorescent Sensor Applications”, J. Am. Chem. Soc., 2002, 124, 11868-11869.
Gaylord et al., “DNA Hybridization Detection with Water-Soluble Conjugated Polymers and Chromophore-Labeled Single-Stranded DNA”, J. Am. Chem. Soc., 2003, 125, 896-900.
Zhou et al., “Fluorescent Chemosensors Based on Energy Migration in Conjugated Polymers: The Molecular Wire Approach to Increased Sensitivity”, J. Am. Chem. Soc., 1995, 117, 12593-12602.
Zhou et al., “Methodology for Enhancing the Sensitivity of Fluorescent Chemosensors: Energy Migration in Conjugated Polymers”, J. Am. Chem. Soc., 1995, 117, 7017-7018.
Hawkins et al., “Incorporation of a fluorescent guanosine analog into oligonucleotides and its application to a rela time assay for HIV-1 integrase 3′-processing reaction”, Nucleic Acids Research, 1995, 23(15), 2872-2880.
Cardullo et al., “Detection of Nucleic Acid Hybridization by Nonradiative Fluorescence Resonance Energy Transfer”, Proc. Natl. Acad. Sci. USA, Dec. 1998, 85, 8790-8794.
Gallot et al., “Poly(L-lysine) containing azobenzene units in the side chains: Influence of the degree of substitution on liquid crystalline structure and thermotropic behaviour”, Liquid Crystals, 1997, 23(1), 137-146.
Wang et al., “Biosnesors from conjugated polyelectrolyte complexes”, PNAS, Jan. 2002, 99(1), 49-53.
Liu et al., “Methods for strand-specific DNA detection with cationic conjugation polymers suitable for incorporation into DNA chips and microarrays”, PNAS Early Edition, Dec. 2004, p. 1-5.
Vehse et al., “Light Amplification by Optical Excitation of a Chemical Defect in a Conjugated Polymer”, Adv. Mater., Jun. 2004, 16(12), 1001-1004.
Liu et al., “Shape-Adapable Water-Soluble Conjugated Polymers”, J. Am. Cham. Soc., 2003, 125, 13306-13307.
Liu et al., “interpolyelectrolyte Complexes of Conjugated Copolymers and DNA: Platforms for Multicolor Biosensors”, J. Am. Chem. Soc., 2004, 126, 1942-1943.
Huang et al., “High-Efficiency, Evironment-Friendly Electroluminescent Polymers with Stable High Work Function Metal as a Cathode: Green- and Yellow-Emitting Conjugated Polyfluorene Polyelectrolytes and Their Neutral Precursors”, J. Am. Chem. Soc., 2004, 126, 9845-9853.
Service, “DNA Analysis: Microchip Arrays Put DNA on the Spot”, The American Association for the Advancement of Science, Oct. 1998, 282(5388), 396-399.
Southern, “DNA chips: analysing sequence by hybridization to oligonucleotides on a large scale”, TIG, Mar. 1996, 12(3), 110-115.
Epstein et al., “Microarray technology—enchanced versatility, persistent challenge”, Current Opinion in Biotechnology, 2000, 11, 36-41.
Heeger et al., “Making Sense of polymer-based biosensors”, PNAS, Oct. 1999, 96(22), 12219-12221.
Patel et al., “Energy transfer analysis of Fos-Jun dimerization and DNA binding”, Proc. Natl. Sci. USA, Jul. 2994, 91, 7360-7364.
Lohse et al., “Fluorescein-Conjugated Lysine Monomers for Solid Phase Synthesis of Fluorescents Peptides and PNA Oligomers”, Bioconjugate Chem., 1997, 8, 503-509.
Smith et al., “The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis”, Nucleic Acids Research, 1985, 13(7) 2399-2412.
Wintermeyer et al., “Fluorescent Derivatives of Yeast tRNA(TM)”, Eur. J. Biochem., 1979, 98, 465-475.
Lipshutz et al., “High density synthetic oligonucleotide arrays”, Nature Genetics Supplement, Jan. 1999, 21, 20-24.
Nilsson et al., “Chip solution detection of DNA hybridization using a luminescent zwitte

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