Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2008-06-03
2008-06-03
Gabel, Gailene R (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S003000, C435S004000, C435S006120, C435S007230, C435S007240, C435S040510, C435S375000, C435S287200, C435S973000, C436S546000, C436S010000, C436S056000, C436S063000, C436S064000, C436S172000, C436S174000
Reexamination Certificate
active
07381535
ABSTRACT:
The invention provides methods and compositions for simultaneously detecting the activation state of a plurality of proteins in single cells using flow cytometry. The invention further provides methods and compositions of screening for bioactive agents capable of coordinately modulating the activity of a plurality of proteins in single cells. The methods and compositions can be used to determine the protein activation profile of a cell for predicting or diagnosing a disease state, and for monitoring treatment of a disease state.
REFERENCES:
patent: 4979824 (1990-12-01), Mathies et al.
patent: 5137809 (1992-08-01), Loken et al.
patent: 5919646 (1999-07-01), Okun et al.
patent: 6280967 (2001-08-01), Ransom et al.
patent: 6379917 (2002-04-01), Okun et al.
patent: 6558916 (2003-05-01), Veerapandian et al.
patent: 6592822 (2003-07-01), Chandler
patent: 6821740 (2004-11-01), Darzynkiewicz et al.
patent: 6958221 (2005-10-01), Veerapandian et al.
patent: 2002/0127604 (2002-09-01), Albritton et al.
patent: 2002/0197658 (2002-12-01), Delaney et al.
patent: 2003/0190688 (2003-10-01), Crosby et al.
patent: 2003/0190689 (2003-10-01), Crosby et al.
patent: 2003/0219827 (2003-11-01), Comb et al.
patent: 2004/0063088 (2004-04-01), Berg et al.
patent: 2004/0072184 (2004-04-01), Yoganathan et al.
patent: 2004/0126784 (2004-07-01), Hitoshi et al.
patent: 2004/0137539 (2004-07-01), Bradford
patent: 2004/0170995 (2004-09-01), Lograsso et al.
patent: 2004/0180380 (2004-09-01), Lee et al.
patent: 2004/0219592 (2004-11-01), Berg et al.
patent: 2004/0224371 (2004-11-01), DeMatos et al.
patent: 2004/0229284 (2004-11-01), Luclw et al.
patent: 2004/0241636 (2004-12-01), Michnick et al.
patent: 2004/0248151 (2004-12-01), Bacus et al.
patent: 2005/0009112 (2005-01-01), Edgar et al.
patent: 2005/0042694 (2005-02-01), Darzynkiewicz et al.
patent: 2005/0074834 (2005-04-01), Chaplen et al.
patent: 2005/0084924 (2005-04-01), Shutts et al.
patent: 2005/0131006 (2005-06-01), Mukherjee et al.
patent: 2005/0216961 (2005-09-01), Delaney
patent: 2005/0250127 (2005-11-01), Fisher et al.
patent: 2005/0281743 (2005-12-01), Delaney
patent: 2006/0029944 (2006-02-01), Huang et al.
patent: 2006/0035211 (2006-02-01), Levinson et al.
patent: 2006/0040338 (2006-02-01), Westwick et al.
patent: 2006/0046249 (2006-03-01), Huang et al.
patent: 2006/0073474 (2006-04-01), Perez et al.
patent: WO 99/44067 (1999-09-01), None
Taylor et al., Complement-Opsonized IgG Antibody/dsDNA Immune Complexes Bind to CR1 Clusters on Isolated Human Erythrocytes (Clinical Immunology and Immunopathology 61: 143-160 (1991).
Bene et al., Detection of Receptor Clustering by Flow Cytometric Fluorescence Anisotropy Measurements (Cytometry 40: 292-306 (2000).
Cochran et al. (Receptor Clustering and transmembrane signaling in T cells, Trends in Biochemical Sciences, 26 (5): May 2001)).
Krutzik et al. “Analysis of Protein Phosphorylation and Cellular Signaling Events by Flow Cytometry: Techniques and Clinical Applications,” Clinical Immunology (2004) 110:206-221.
Pettersen et al. “CD47 Signals T Cell Death,” The American Association of Immunologists (1999) -162:7031-7040.
Allende, L. M., et al., (2000). “A novel CD18 genomic deletion in a patient with severe leucocyte adhesion deficiency: a possible CD2/lymphocyte function-associated antigen-1 functional association in humans.” Immunology 99:440-50.
Anderson, M. T. et al. “Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins.” Proc Natl Acad Sci U S A 93, 8508-11 (1996).
Bleijs, D. A., et al. (2001). “A single amino acid in the cytoplasmic domain of the beta 2 integrin lymphocyte function-associated antigen-1 regulates avidity-dependent inside-out signaling.” J Biol Chem 276, 10338-10346.
Cairo, C.W., et al., “Control of Multivalent Interactions by Binding Epitope Density”, J. Am. Chem. Soc., 2002, 124(8)1615-1619.
Cochran, J.R., et al., “Receptor clustering and transmembrane signaling in T cells”, Trends in Biochemical Sciences, May 2001, 26(5)304-310.
Colucci, F., et al. (1999). “Redundant role of the Syk protein tyrosine kinase in mouse NK cell differentiation.” J Immunol 163, 1769-1774.
Damle, N. K., et al. (1992c). “Differential regulatory effects of intercellular adhesion molecule-1 on costimulation by the CD28 counter-receptor B7.”J Immunol 149, 2541-2548.
Dantuma, N. P., et al. “Short-lived green fluorescent proteins for quantifying ubiquitin/proteasome-dependent proteolysis in living cells.” Nat Biotechnol 18, 538-43. (2000).
Davis, K. A., et al. (1998). “Determination of CD4 antigen density on cells: role of antibody valency, avidity, clones, and conjugation.” Cytometry 33, 197-205.
De Fougerolles, A. R., et al. (1995). “Heterogenous glycosylation of ICAM-3 and lack of interaction with Mac-1 and p. 150,95.” Eur J Immunol 25, 1008-12.
De Fougerolles, et al. (1991). “Characterization of ICAM-2 and evidence for a third counter-receptor for LFA-1.” J Exp Med 174, 253-67.
De Rosa, S. C., et al. “11-color, 13-parameter flow cytometry: Identification of human naive T cells by phenotype, function, and T-cell receptor diversity.” Nat Med 7, 245-248. (2001).
Deeths, M. J., and Mescher, M. F. (1999). “ICAM-1 and B7-1 provide similar but distinct costimulation for CD8+ T cells, while CD4+ T cells are poorly costimulated by ICAM-1.” Eur J Immunol 29, 45-53.
Devine, L., et al. “Role of LFA-1, ICAM-1, VLA4 and VCAM-1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro.” Immunology 88, 456-62.
Diacovo, T. G., et al. (1994). “A functional integrin ligand on the surface of platelets: intercellular adhesion molecule-2.” J Clin Invest 94, 1243-51.
Dikic, I., et al., (1996). “A role for Pyk2 and Src in linking G-protein-coupled receptors with MAP kinase activation.” Nature 383, 547-50.
Donskov, F., et al. (1996). “Expression and function of LFA-1 and A-NK and T-LAK cells: role in tumor target killing and migration into tumor tissue.”Nat Immun 15, 134-146.
Fiering, S. N. et al. “Improved FAGS-Gal: flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs.” Cytometry 12, 291-301. (1991).
Fine, J. S., and Kruisbeek, A. M. (1991). “The role of LFA-1/ICAM-1 interactions during murine T lymphocyte development.” J Immunol 147, 2852-2859.
Geiger, C., et al. (2000). “Cytohesin-1 regulates beta-2 integrin-mediated adhesion through both ARF-GEF function and interaction with LFA-1.” Embo J 19, 2525-2536.
Griffioen, A. W., et al. (1996). “Endothelial intercellular adhesion molecule-1 expression is suppressed in human malignancies: the role of angiogenic factors.”Cancer Res 56, 1111-17.
Griffioen, A. W., et al. (1996). “Tumor angiogenesis is accompanied by a decreased inflammatory response of tumor-associated endothelium.” Blood 88,667-73.
Haswell, L.E., et al., “Analysis of the oligomeric requirement for signaling by CD40 using soluble multimeric forms of its ligands, CD154”, Eur. J. Immunol., 2001, 31(10):3094-3100.
Helander, T. S., et al. (1996). :ICAM-2 redistributed by ezrin as a target for killer cells. Nature 382, 265-8.
Hogg, N., et al. (1999). “A novel leukocyte adhesion deficiency caused by expressed but nonfunctional beta2 integrins Mac-1 and LFA-1.” J Clin Invest 103, 97-106.
Igietseme, J. U., et a. (1999). “The intercellular adhesion molecule type-1 is required for rapid activation of T helper type 1 lymphocytes that control early acute phase of genital chlamydial infection in mice.” Immunology 98, 510-8.
Iyer, S. B., et al. (1998). “Quantitation of CD38 expression using QuantiBRITE™ beads.” Cytometry 33, 206-12.
Jiang, K., et al. (2000). “Pivotal
Nolan Garry P.
Perez Omar D.
Bozicevic Field & Francis LLP
Field Bret E.
Gabel Gailene R
Scherer David C.
The Board of Trustees of the Leland Stanford Junior
LandOfFree
Methods and compositions for detecting receptor-ligand... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods and compositions for detecting receptor-ligand..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for detecting receptor-ligand... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2812462