Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1993-11-15
1996-12-31
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 911, 435 912, 435196, 435199, 435810, 436501, 530350, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 78, 935 88, C12Q 168
Patent
active
055893295
ABSTRACT:
The present invention discloses improved compositions and methods for detecting mutations, including single base changes, in nucleic acid sequences using RNase protection assays. The improvements include concomitant, dramatic reductions in the salt and RNase enzyme concentrations in the RNase digestion reaction mixture which result in greater sensitivity in detecting genetic mutations. Another embodiment of the present invention is kits to be used for the detection of single base mismatches in nucleic acid samples.
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Goldrick Marianna M.
Winkler Matthew
Ambion Inc.
Jones W. Gary
Marschel Ardin H.
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