Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2004-09-24
2008-11-11
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S024330
Reexamination Certificate
active
07449293
ABSTRACT:
The invention provides methods and compositions for rapid, sensitive, and highly specific nucleic acid-based detection ofB. anthracisin a sample. In general, the methods involve detecting within the sspE gene ofB. anthracisthe presence of a fragment of six unique contiguous nucleotides that is not present in the sspE genes of otherBacillusbacteria or other non-Bacillusbacteria. In many embodiments, the methods involve amplifying a nucleic acid comprising at least a fragment of an sspE gene from a sample, and detecting the presence of the six unique contiguous nucleotide fragments in the nucleic acid. The invention also provides primers and kits for detection ofB. anthracisin a sample. The subject invention finds use in a variety of different applications, including research, medical, diagnostic and military applications.
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Kim Kijeong
Leighton Terrance
Bertagna Angela
Bozicevic Field & Francis LLP
Children's Hospital & Research Center at Oakland
Horlick Kenneth R.
Keddie James S.
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