Methods and compositions for detecting and treating mycobacteria

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 232, 536 237, 530350, 530825, 4351723, C12N 1500, C07H 2104, C07K 1400

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056865902

ABSTRACT:
The embodiments of the invention are based upon the identification and characterization of genes that determine mycobacterial resistance to the antibiotic isoniazid (INH) and its analogs. These genes, termed inhA, encode a polypeptide, InhA, that is the target of action of mycobacteria for isoniazid. The sequences of wild-type INH-sensitive as well as allelic or mutant INH-resistant inhA genes and their operons are provided. Also provided are isolated InhA polypeptides of both the INH-resistant and INH-sensitive types.

REFERENCES:
patent: 5573915 (1996-11-01), Barry
Banerjee et al., "inhA, a gene encoding a target for isoniazid and ethionde in Mycobacterium tuberculosis" Science (1994) 263:277-230.
Uhlmann et al., "Antisense oligonucleotides: A new therapeutic principle" Chem. Reviews (1990) 90(4):543-584.
Orita et al., "Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction" Genomics (1989) 5:874-879.
Telenti et al., "Rapid identification of Mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis" J. Clin. Microbiol. (1993) 31(2):175-178.
Zhang et al Nature 358 591-593, 1992.

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