Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-08-26
1995-08-29
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 6, 436501, 436506, 436512, 436548, C12Q 168, G01N 33564, G01N 33577
Patent
active
054459400
ABSTRACT:
Provided are monoclonal antibodies, fragments, and derivatives thereof reactive with an epitope of the T cell receptor alpha chain variable region, V.alpha.12.1, on human T lymphocytes. The monoclonal antibodies are reactive with approximately 2% of CD4.sup.+ T lymphocytes and with approximately 5% of CD8.sup.+ T lymphocytes in peripheral blood cells in normal individuals and define a subset of individuals afflicted with an autoimmune disease, especially rheumatoid arthritis, that exhibit increased expression of the V.alpha.12.1 gene on CD8.sup.+ peripheral blood T lymphocytes when compared to normal individuals. Also provided are methods for diagnosing, treating, and monitoring the progression of rheumatoid arthritis in a subject using V.alpha.12.1-specific reagents, including antibodies and nucleic acid probes. Higher levels of assurance in the diagnosis of RA can be made by establishing that the expansion of V.alpha.12.1 gene usage is clonal or oligoclonal and that the V.alpha.12.1 expansion correlates with the occurrence of the MHC allele, HLA-DQw2.
REFERENCES:
patent: 4550086 (1985-10-01), Reinherz et al.
patent: 4657760 (1987-04-01), Kung et al.
patent: 4713332 (1987-12-01), Mak
patent: 4845026 (1989-07-01), Kung et al.
patent: 4886743 (1989-12-01), Hood et al.
patent: 4923799 (1990-05-01), Mak
patent: 5024940 (1991-06-01), Brenner et al.
patent: 5034316 (1991-07-01), Weisbart
J. D. Fraser et al., "A solubilized T-cell receptorfrom a human leukemia cell linebinds to a ligand in the absence of MHC products," Immunogenetics, 28:108-116 (1989).
Janson et al, "Predominant T. Cell Receptor V Gene Usage in Patients With Abnormal Clones of B Cells", Blood, 77 (Apr., 15, 1991).
Janson et al, "Abnormal T Cell Expansion and V-Gene Usage In Patients With Myasthenia Gravis Multiple Myeloma And MGUS," European Immunology Meeting Abstract, Jun. 10-12, (1991).
B. Kotzin et al, "T cell repertoire abnormalities in rheumatoid arthritis," J. Cellular Biochem., suppl. 15 (subpart E), p. 144 (Mar. 1991).
Grunewald et al, "Abnormal T Cell expansion and V-Gene usage in Myasthenia Gravis Patients", Scaninavian J. Immunol., 34:161-168.
Brenner et al, "Characterization And Expression Of The Human aB T Cell Receptor By Using A Framework Monoclonal Antibody", J. Immunol., 138:1503-1508 (Mar. 1987).
Sim et al, "Primary Structure of human T-cell receptor a-chain", Nature, 312:771-775 (Dec. 1984).
Brenner et al, "Identification of Shared Antigenic Determinants Of The Putative Human T Lymphocyte Antigen Receptor", J. Exp. Med., 160:541-551 (Aug. 1984).
Janson et al, "Anti-idiotypic monoclonal antibody to a T-cell chronic lymphatic leukemia", Cancer Immunol. Immunother., 28:225-232 (1989).
Utsunomiya et al, "Analysis Of A Monoclonal Rat Antibody Directed To The a-Chain Variable Region (Va3) Of the Mouse T Cell Antigen Receptor", J. Immunol., 143:2602-2608 (Oct. 15, 1981).
Jameson et al, "A T Cell Receptor Va Region Selectively Expressed in CD4.sup.+ Cells", J. Immunol., 145:1324-1331 (Sep. 1, 1990.
Hannun et al, "Immunoglobulin-Like Nature Of A Human T-Cell Antigen/MHC Receptor," Nature, 312:65-67 (Nov. 1984).
Yoshikai et al, "Sequences and Repertoire Of Human T Cell Receptor a Chain Variable REgion Genes In Mature T Lymphocytes," J. Exp. Med., 164:90-103 (Jul. 1986).
Kimura et al, "Sequences and repertoire of the human T cell receptor a and B chain variable region genes in thymocytes," Eur. J. Immunol., 17:375-383 (1987).
Klein et al, "Diversity and structure of human T-cell receptor a-chain variable region genes," Proc. Natl. Acad. Sci., USA, 84:6884-6888 (Oct. 1987).
Roman-Roman et al, "Studies on the human T cell receptor a/B variable region genes, I. Identification of 7 additional Va subfamilies and 14 Ja gene segments," Eur. J. Immunol., 21:927-933 (1991).
Posnettt et al, "A Novel Method for Producing Anti-Peptide Antibodies," J. Biol. Chem., 263 (4):1719-1725 (1988).
Boylston et al, "Properties Of A Panel Of Monoclonal Antibodies Which React With The Human T Cell Antigen Receptor On The Leukemic Line HPB-ALL And A Subset Of Normal Peripheral Blood T Lymphocytes," J. Immunol., 137(2):741-744 (Jul. 15, 1986).
Galeazzi M. et al, "HLA-DR association in rheumatoid arthritis and the shared susceptibility epitope hypothesis" Immunology Letters 663-664.
Cappellacci et al, "HLA antigens and adult rheumatoid arthritis: a study with a monoclonal antibody", Clin. and Exp. Pheu., 5:63-66 (1987).
Tait and Simons, Detection of Immune-Associated Genetic Markers of Human Disease, Chapters 4-6 (Church 11 Livingston, pub.) (1985).
H. Dersimonian et al, Jour. Exp. Med., 174, 639-648, 1991.
L. E. Hood et al, Immunology, Second Edition, The Benjamin/Cummings Publishing Company, Inc., 1984, pp. 66-68.
Brenner Michael B.
Der Simonian Harout
Brigham & Women's Hospital
Dana-Farber Cancer Institute
Hart Julia D.
Saunders David
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