Methods and compositions for cyclizing diterpenes

Details Methods and compositions for cyclizing diterpenes Methods and compositions for cyclizing diterpenes

2004-03-11

2008-03-04

Ramirez, Delia M. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

C435S004000, C435S183000, C530S350000

Reexamination Certificate

active

07338793

ABSTRACT:
An enzyme having diterpene cyclase activity has been purified fromP. elisabethaeusing a series of chromatography steps. The purified enzyme has an apparent molecular weight of about 47 kilodaltons and an isoelectric point of about 5.1. The purifed enzyme catalyzed the cyclization of geranyl geranyl diphosphate to elisabethatriene.

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