Methods and compositions for cellular and metabolic engineering

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification

Reexamination Certificate

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C435S006120, C536S022100, C536S023100, C536S024300

Reexamination Certificate

active

08048674

ABSTRACT:
The present invention is generally directed to the evolution of new metabolic pathways and the enhancement of bioprocessing through a process herein termed recursive sequence recombination. Recursive sequence recombination entails performing iterative cycles of recombination and screening or selection to “evolve” individual genes, whole plasmids or viruses, multigene clusters, or even whole genomes. Such techniques do not require the extensive analysis and computation required by conventional methods for metabolic engineering.

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