Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Destruction of hazardous or toxic waste
Patent
1998-09-02
2000-08-01
Redding, David A.
Chemistry: molecular biology and microbiology
Process of utilizing an enzyme or micro-organism to destroy...
Destruction of hazardous or toxic waste
B09B 300
Patent
active
060965312
ABSTRACT:
Disclosed are methods for assembling a consortium of specific degraders of a xenobiotic compound of interest. A first population of microorganisms is provided, the first population being isolated from a first source containing the xenobiotic compound of interest. The first population of microorganisms is then incubated under conditions appropriate for growth with media containing the xenobiotic compound of interest as a carbon source. The population of then screened for the ability to initiate degradation of the xenobiotic compound of interest. Accumulating intermediate are identified in the degradation pathway of the xenobiotic compound of interest. A second population of microorganisms is then isolated from a source containing the accumulating intermediate in the xenobiotic degradation pathway, or structural homologs thereof. The second population of microorganisms is then incubated under conditions appropriate for growth with media containing the accumulating intermediate as a carbon source. Screening for the ability of the second population of microorganisms to degrade the accumulating intermediate is then carried out. Optionally, the steps recited above can be repeated to identify additional accumulating intermediates, and populations of microorganisms which specifically degrade such additional accumulating intermediates. The populations identified in this manner are then combined under conditions appropriate for the integration of the populations into a single consortium of specific degraders of the xenobiotic compound of interest. Consortia assembled by these methods can then be employed in connection with methods for degrading a xenobiotic compound of interest.
REFERENCES:
patent: 5484729 (1996-01-01), DeWeerd et al.
patent: 5932472 (1999-08-01), Abdullah
patent: 5955350 (1999-09-01), Soni et al.
Farrell Kevin M.
Green Mountain Laboratories, Inc.
Redding David A.
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