Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1980-11-24
1983-09-13
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435188, 435810, 436518, 436537, 436547, 436808, G01N 3354, C12N 996
Patent
active
044042787
ABSTRACT:
Methods and compositions for assaying for coenzymes having N-substituted 1,4-dihydropyridyl as the active portion of the prosthetic group. The conversion of NAD to NADH is widely measured both for determining NAD-NADH (including the analogs thereof) dependent enzymes and enzymes that can be coupled to NAD-NADH dependent enzymes, and for determining ligands or receptors in competitive protein binding assays. Antibodies specific for NADH in the presence of NAD are employed, providing enhanced fluorescence over the normal fluorescence of unbound NADH. By determining the rate of formation of NADH or the concentration of NADH, the analyte or enzyme of interest can be determined.
REFERENCES:
patent: 4067774 (1978-01-01), Rubenstein
patent: 4134792 (1979-01-01), Boguslaski et al.
patent: 4233402 (1980-11-01), Maggio et al.
patent: 4238565 (1980-12-01), Hornby et al.
patent: 4252783 (1981-02-01), Kam et al.
patent: 4318707 (1982-03-01), Litman et al.
patent: 4341866 (1982-07-01), Yoshida et al.
Parker, Radioimmunoassay of Biologically Active Compounds, Prentice-Hall, Inc., Englewood Cliffs, (1976), pp.36-67.
Kanemitsu, "Antibodies to Coenzymes", Chem. Absts., vol. 93, No. 24 (1980), pp. 315-316, Absts. No. 225617s.
Bredehorst, "Determination of radioimmunoassay of the Sum of Oxidized and reduced forms of NAD and NADP in picomole quantities from the same Acid Extract" Chem. Absts. vol. 91, No. 21 (1979) pp. 274-275.
Ogievetskaya et al., "Study of the Localization of Adenine Components in Chicken Myofibers by the Ferritin Antibody Method" Chem. Absts., vol. 90, No. 17, (1979), pp. 295-296, Absts. No. 135336t.
Schrader et al., "Radioimmunoassay for Adenosine in Biological Samples", Chem. Absts. vol 90, No. 11, (1979) p. 264, Absts. No. 83207e.
Bredehorst et al., "Production of Antibodies Against ADP-Ribose and 5'-AMP with aid of N.sup.6 -Carboxy Methylated ADP-Ribose Conjugates", Chem. Absts., vol. 88, No. 13 (1978), p. 199, Absts. No. 85510f.
Colvin Floyd W.
Hu Mae W.
Ullman Edwin F.
Rowland Bertram I.
Syva Company
Wiseman Thomas G.
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