Methods and compositions for analyzing AHASL genes

Details Methods and compositions for analyzing AHASL genes Methods and compositions for analyzing AHASL genes

2004-03-22

2008-10-07

Sitton, Jehanne (Department: 1634)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C536S023100

Reexamination Certificate

active

07432082

ABSTRACT:
The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes ofTriticum aestivumand the two AHASL genes ofTriticum turgidumssp.durum.

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