Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-09-20
2005-09-20
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200
Reexamination Certificate
active
06946251
ABSTRACT:
The invention provides methods for isothermal amplification of RNA. The methods are particularly suitable for amplifying a plurality of RNA species in a sample. The methods employ a composite primer, a second primer and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In another aspect, the methods employ a single primer (which is a composite primer) and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In some embodiments, a transcription step is included to generate multiple copies of sense RNA of an RNA sequence of interest. The methods are useful for preparation of nucleic acid libraries and substrates for analysis of gene expression of cells in biological samples. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
REFERENCES:
patent: 4582788 (1986-04-01), Erlich
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4786600 (1988-11-01), Kramer et al.
patent: 4876187 (1989-10-01), Duck et al.
patent: 4908385 (1990-03-01), Bar-Tana et al.
patent: 5011769 (1991-04-01), Duck et al.
patent: 5043272 (1991-08-01), Hartley
patent: 5106727 (1992-04-01), Hartley et al.
patent: 5130238 (1992-07-01), Malek et al.
patent: 5169766 (1992-12-01), Schuster et al.
patent: 5185243 (1993-02-01), Ullman et al.
patent: 5194370 (1993-03-01), Berninger et al.
patent: 5270184 (1993-12-01), Walker et al.
patent: 5399491 (1995-03-01), Kacian et al.
patent: 5403711 (1995-04-01), Walder et al.
patent: 5409818 (1995-04-01), Davey et al.
patent: 5427911 (1995-06-01), Ruano
patent: 5427929 (1995-06-01), Richards et al.
patent: 5437990 (1995-08-01), Burg et al.
patent: 5455166 (1995-10-01), Walker
patent: 5480784 (1996-01-01), Kacian et al.
patent: 5508178 (1996-04-01), Rose et al.
patent: 5510270 (1996-04-01), Fodor et al.
patent: 5545522 (1996-08-01), Van Gelder et al.
patent: 5554516 (1996-09-01), Kacian et al.
patent: 5554517 (1996-09-01), Davey et al.
patent: 5556752 (1996-09-01), Lockhart et al.
patent: 5578832 (1996-11-01), Trulson et al.
patent: 5589339 (1996-12-01), Hampson et al.
patent: 5595891 (1997-01-01), Rose et al.
patent: 5648211 (1997-07-01), Fraiser et al.
patent: 5654142 (1997-08-01), Kievits et al.
patent: 5660988 (1997-08-01), Duck et al.
patent: 5665545 (1997-09-01), Malek et al.
patent: 5679512 (1997-10-01), Laney et al.
patent: 5683879 (1997-11-01), Laney et al.
patent: 5700642 (1997-12-01), Monforte et al.
patent: 5710028 (1998-01-01), Eyal et al.
patent: 5712124 (1998-01-01), Walker
patent: 5716785 (1998-02-01), Van Gelder et al.
patent: 5731146 (1998-03-01), Duck et al.
patent: 5744308 (1998-04-01), Guillou-Bonnici et al.
patent: 5744312 (1998-04-01), Mamone et al.
patent: 5747255 (1998-05-01), Brenner
patent: 5763178 (1998-06-01), Chirikjian et al.
patent: 5766849 (1998-06-01), McDonough et al.
patent: 5773601 (1998-06-01), Agrawal
patent: 5824517 (1998-10-01), Cleuziat et al.
patent: 5824518 (1998-10-01), Kacian et al.
patent: 5829547 (1998-11-01), Fujii et al.
patent: 5830655 (1998-11-01), Monforte et al.
patent: 5846710 (1998-12-01), Bajaj
patent: 5849547 (1998-12-01), Cleuziat et al.
patent: 5854033 (1998-12-01), Lizardi
patent: 5858665 (1999-01-01), Hepp et al.
patent: 5871697 (1999-02-01), Rothberg et al.
patent: 5876976 (1999-03-01), Richards et al.
patent: 5882867 (1999-03-01), Ullman et al.
patent: 5888779 (1999-03-01), Kacian et al.
patent: 5888819 (1999-03-01), Goelet et al.
patent: 5916777 (1999-06-01), Kacian et al.
patent: 5932450 (1999-08-01), Dattagupta et al.
patent: 5958681 (1999-09-01), Wetmur et al.
patent: 5962271 (1999-10-01), Chenchik et al.
patent: 5962272 (1999-10-01), Chenchik et al.
patent: 5965409 (1999-10-01), Pardee et al.
patent: 6004744 (1999-12-01), Goelet et al.
patent: 6004745 (1999-12-01), Arnold, Jr. et al.
patent: 6013431 (2000-01-01), Soderlund et al.
patent: 6027889 (2000-02-01), Barany et al.
patent: 6027923 (2000-02-01), Wallace
patent: 6030774 (2000-02-01), Laney et al.
patent: 6037152 (2000-03-01), Richards et al.
patent: 6090591 (2000-07-01), Burg et al.
patent: 6096715 (2000-08-01), Rossi et al.
patent: 6107032 (2000-08-01), Kilger et al.
patent: 6132997 (2000-10-01), Shannon
patent: 6136533 (2000-10-01), Bekkaoui et al.
patent: 6143495 (2000-11-01), Lizardi et al.
patent: 6218151 (2001-04-01), Cleuziat et al.
patent: 6251600 (2001-06-01), Winger et al.
patent: 6251639 (2001-06-01), Kurn
patent: 6291170 (2001-09-01), Van Gelder et al.
patent: 6358712 (2002-03-01), Jarrell et al.
patent: 6686156 (2004-02-01), Kurn
patent: 6692918 (2004-02-01), Kurn
patent: 6815164 (2004-11-01), Kurn
patent: 2001/0000077 (2001-03-01), Engelhardt et al.
patent: 2001/0034048 (2001-10-01), Kurn
patent: 2001/0041334 (2001-11-01), Rashtchian et al.
patent: 2002/0058270 (2002-05-01), Kurn
patent: 2002/0115088 (2002-08-01), Kurn
patent: 2002/0127575 (2002-09-01), Hoke et al.
patent: 2002/0142309 (2002-10-01), Dattagupta
patent: 2002/0164628 (2002-11-01), Kurn
patent: 2002/0177141 (2002-11-01), Chee et al.
patent: 2003/0017591 (2003-01-01), Kurn
patent: 2003/0049657 (2003-03-01), Cherry
patent: 2003/0073081 (2003-04-01), Mukai et al.
patent: 2003/0104460 (2003-06-01), Rabbani et al.
patent: 2003/0186234 (2003-10-01), Kurn
patent: 2003/0215926 (2003-11-01), Kurn et al.
patent: 2004/0023271 (2004-02-01), Kurn et al.
patent: 0 050 424 (1982-04-01), None
patent: 0 084 796 (1983-08-01), None
patent: 0 201 184 (1986-12-01), None
patent: 0 237 362 (1987-09-01), None
patent: 0 258 017 (1988-03-01), None
patent: 0 320 308 (1989-06-01), None
patent: 0 365 627 (1990-05-01), None
patent: 0 395 398 (1990-10-01), None
patent: 0 395 398 (1990-10-01), None
patent: 0 497 272 (1992-08-01), None
patent: 0 497 272 (1992-08-01), None
patent: 0 500 224 (1992-08-01), None
patent: 0 500 224 (1992-08-01), None
patent: 0 505 012 (1992-09-01), None
patent: 0 543 612 (1993-05-01), None
patent: 0 543 612 (1993-05-01), None
patent: 0 543 612 (1993-05-01), None
patent: 0 667 393 (1995-08-01), None
patent: 0 667 393 (1995-08-01), None
patent: 0 878 553 (1998-11-01), None
patent: 0 971 039 (2000-01-01), None
patent: 1 055 736 (2000-11-01), None
patent: 1 167 524 (2002-01-01), None
patent: 1 273 737 (2003-01-01), None
patent: 1 281 757 (2003-02-01), None
patent: 1 312 682 (2003-05-01), None
patent: 06-327500 (1994-11-01), None
patent: 07-023799 (1995-01-01), None
patent: WO 88/02746 (1988-04-01), None
patent: WO 88/10315 (1988-12-01), None
patent: WO 89/01050 (1989-02-01), None
patent: WO 92/15712 (1992-09-01), None
patent: WO 95/03426 (1995-02-01), None
patent: WO 97/04126 (1997-02-01), None
patent: WO 97/32040 (1997-09-01), None
patent: WO 98/01050 (1998-01-01), None
patent: WO 98/28443 (1998-07-01), None
patent: WO 99/18241 (1999-04-01), None
patent: WO 99/29901 (1999-06-01), None
patent: WO 99/37808 (1999-07-01), None
patent: WO 99/40219 (1999-08-01), None
patent: WO 99/42618 (1999-08-01), None
patent: WO 99/55912 (1999-11-01), None
patent: WO 00/08208 (2000-02-01), None
patent: WO 00/08208 (2000-02-01), None
patent: WO 00/09745 (2000-02-01), None
patent: WO 00/28082 (2000-05-01), None
patent: WO 00/40715 (2000-07-01), None
patent: WO 00/52191 (2000-09-01), None
patent: WO 00/56877 (2000-09-01), None
patent: WO 00/56925 (2000-09-01), None
patent: WO 00/70095 (2000-11-01), None
patent: WO 02/29117 (2001-02-01), None
patent: WO 01/20035 (2001-03-01), None
patent: WO 01/23613 (2001-04-01), None
patent: WO 01/64952 (2001-09-01), None
patent: WO 01/64952 (2001-09-01), None
patent: WO 01/73134 (2001-10-01), None
patent: WO 02/00938 (2002-01-01), None
patent: WO 02/28876 (2002-04-01), None
patent: WO 02/28876 (2002-04-01), None
patent: WO 02/48402 (2002-06-01), None
patent: WO 02/057487 (2002-07-01), None
patent: WO 02/072772 (2002-09-01), None
patent: WO 02/072773 (2002-09-01), None
patent: WO 02/103013 (2002-12-01), None
patent: WO 03/012100 (2003-02-01), None
patent: WO 03/012142 (2003-02-01), None
patent: WO 03/078645 (2003-09-01), Non
Horlick Kenneth R.
NuGEN Technologies, Inc.
LandOfFree
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