Methods and composition for the production of orthogonal...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...

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C435S193000, C435S091400, C435S252300, C435S252330, C435S320100, C435S069100, C435S254110, C435S254200, C536S023200

Reexamination Certificate

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11254170

ABSTRACT:
This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.

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