Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2006-05-30
2006-05-30
Mehta, Ashwin (Department: 1638)
Chemistry: molecular biology and microbiology
Vector, per se
C435S468000, C800S278000
Reexamination Certificate
active
07052905
ABSTRACT:
The present invention provides a method and composition for expression of multiple genes from a polycistronic message in transgenic plants using genetic elements derived from the peanut chlorotic streak caulimovirus promoter-leader sequence and antisense sequence of PC1SV ORF VII. Also provided are compositions and methods for intron-mediated enhanced and regulated expression of genes in transgenic plants.
REFERENCES:
I.B. Maiti et al., “Isolation and Expression Analysis of Peanut Chlorotic Streak Caulimovirus (PCISV) Full-Length Transcript (FLT) Promoter in Transgenic Plants”, Biochemical and Biophysical Research Communications, vol. 244, (1998), pp. 440-444.
I.B. Maiti et al., “Promoter/leader deletion analysis and plant expression vectors with the figwort mosaic virus (FMV) full length transcript (FLt) promoter containing single or double enhancer domains”, Transgenic Research 6, (1997), pp. 143-156.
I.B. Maiti et al., “Gene expression regulated by gene VI of caulimovirus: transactivation of downstream genes of transcripts by gene VI of peanut chlorotic streak virus in transgenic tobacco”, Virus Research, vol. 57, (1998), pp. 113-124.
S. Bhattacharyya et al., Intron-mediated enhancement of gene expression in transgenic plants using chimeric constructs composed of thePeanut chlorotic streak virus(PCISV) promoter-leader and the antisense orientation of PCISV ORF VII (p7R), Plant, vol. 218, (2003), pp. 115-124.
I.B. Maiti et al., “Developing Genetically Engineered Disease, Pest and Herbicide Resistance in Tobacco”, 46th Tobacco Chemists' Research Conference, Recent Advances in Tobacco Science, Highlights of Current Research on Tobacco and Tobacco Chemistry, Proceedings of a Symposium Presented at the 46th Meeting of the Tobacco Chemists' Research Conference, vol. 18, Sep. 27-30, 1992, pp. 45-68.
G. Wagner, “Improving Tobacco Through Metabolic Engineering: Promise and Obstacles”, 46th Tobacco Chemists' Research Conference, Recent Advances in Tobacco Science, Highlights of Current Research on Tobacco and Tobacco Chemistry, Proceedings of a Symposium Presented at the 46th Meeting of the Tobacco Chemists' Research Conference, vol. 18, Sep. 27-30, 1992, pp. 3-43.
Bhattacharyya Somnath
Maiti Indu Bhushan
McDermott Will & Emery LLP
Mehta Ashwin
University of Kentucky Research Foundation
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