Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1983-01-13
1986-03-04
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351722, 4351723, 435240, 435948, 435173, 935 96, 935100, 935106, C12P 2100, C12N 1500, C12N 1300, C12N 500
Patent
active
045741166
ABSTRACT:
Methods are provided for producing fusion partners which involve employing an immortalized human myeloma cell line sensitive to HAT and having an additional dominant selectable resistance marker and fusing the doubly marked human myeloma cells with a stable immortalized rodent myeloma cell line, desirably previously subjected to substantial chromosome damage, and isolating cells having a substantially complete chromosomal complement of the rodent cell and at least about one chromosome of the human cell having a gene expressing said resistance, thereby being resistant to a selective agent. The resulting heteromyeloma may be fused with high efficiency with human lymphocytes to produce monoclonal antibodies.
The cell lines designated as A6 and 36 were deposited at the A.T.C.C. on Jan. 11, 1983 and given accession numbers CRL8192 and CRL8193, respectively.
REFERENCES:
Kozbor et al, "Human Anti Tetanus Toxoid Monoclonal Antibody Secreted by EBV-Transformed Human B Cells Fused with Murine Myeloma", Hybridoma, 1(3), pp. 323-328 (1982).
Croce et al, "Preferential Retention of Human Chromosone 14 in Mouse X Human B Cell Hybrids", European Journal of Immunology 10, pp. 486-488 (1980).
Tucker et al, "Specific Bovine Monoclonal Antibody Produced by a Re-Fused Mouse/Calf Hybridoma", Hybridoma 3(2), pp. 171-176 (1984).
Ostberg et al, "Human X (Mouse X Human) Hybridomas Stably Producing Human Antibodies", Hybridoma 2(4), pp. 361-367 (1983).
Levy et al, "Rescue of Immunoglobulin Secretion from Human Neoplastic Lymphoid Cells by Somatic Cell Hybridization", Proceedings of the National Academy of Sciences, 75(5), pp. 2411-2415 (1978).
Nowinski et al, "Human Monoclonal Antibody Against Forssman Antigen", Science 210, pp. 537-539 (1980).
Olsson et al, "Human Human Hybridomas Producing Monoclonal Antibodies of Predefined Antigenic Specificity", Proceedings of the National Academy of Sciences, 77(9), pp. 5429-5431 (1980).
Shulman et al, "A Better Cell Line for Making Hybridomas Secreting Specific Antibodies",Nature, 276, pp. 269-270 (1978).
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Littlefield, "Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed Recombinants", Science, 145, pp. 709-710 (1964).
Wright, "The Selection of Heterokaryons and Cell Hybrids using the Biochemical Inhibitors Iodoacetamide and Diethylpyrocarbonate", Techniques in Somatic Cell Genetics ed, Shay, Plenum Press, NY, pp. 47-65 (1982).
Fazekas et al, "Production of Monoclonal Antibodies: Strategy and Tactics",Journal of Immunological Methods, 35, pp. 1-21 (1980).
Calvo-Riera Francisco
Kaplan Henry S.
Lam Kit S.
Teng Nelson N. H.
Rowland Bertram I.
Tanenholtz Alvin E.
Tarcza John Edward
The Board of Trustees of the Leland Stanford Jr. University
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