Methods and apparatuses of separating cells using magnets...

Details Methods and apparatuses of separating cells using magnets... Methods and apparatuses of separating cells using magnets...

2007-03-13

2007-03-13

Beisner, William H. (Department: 1744)

Chemistry: molecular biology and microbiology

Apparatus

Means for separation or recovery of a microorganism from...

C435S287200, C435S288500, C422S073000, C436S063000, C436S526000

Reexamination Certificate

active

10878357

ABSTRACT:
Disclosed are methods and apparatuses for separating cells using magnets and droplet type cell suspension according to the present invention, which may effectively separate cells by forming droplet type cell suspension with cell suspension sample containing cells to which magnetic beads are coupled and applying magnetic force to the droplet type cell suspension.The apparatus of separating cells according to the present invention includes: a droplet type cell suspension forming part for forming a droplet type cell suspension under a lower part of the droplet type cell suspension forming part with cell suspension sample containing cells to which magnetic beads are coupled; a cell suspension inlet for supplying the droplet type cell suspension forming part with the cell suspension sample; a magnet for applying magnetic force to the droplet type cell suspension; a cell buffer inlet for supplying the droplet type cell suspension with cell buffer; and a temperature maintaining part for maintaining a temperature of the droplet type cell suspension.

REFERENCES:
patent: 5646001 (1997-07-01), Terstappen et al.
Rong Rong et al., “A Functional Magnetic Bead/Biocell Sorter Using Fully Integrated Magnetic Micro/Nano Tips,” IEEE the 16thAnnual International Conference on MEMS, Jan. 19-23, 2003; pp. 530-533, 03CH37426.
Tao Deng et al., “Fabrication of Magnetic Microfiltration Systems Using Soft Lithography,” Applied Physics Letters, vol. 80, No. 3; Jan. 21, 2002; pp. 461-463.
Michael Berger et al., “Design of a Microfabricated Magnetic Cell Separator,” Electrophoresis, vol. 22, No. 16, Oct. 2001; pp. 3883-3892.

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