Methods and apparatus for sequencing polymers using mass spectro

Details Methods and apparatus for sequencing polymers using mass spectro Methods and apparatus for sequencing polymers using mass spectro

1997-02-07

1998-10-27

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 4352872, 4352887, 435 4, 435 911, 435173, 422 99, 530333, 530300, C12Q 168, C12Q 170, C12P 1934, C07K 500

Patent

active

058276599

ABSTRACT:
The methods and apparatus disclosed herein are useful for sequencing polymers using mass spectrometry. The methods involve differing ratios of hydrolyzing agent to polymer disposed upon a reaction surface adapted for use with a mass spectrometer. The methods further involve integrating data obtained from mass spectrometry analysis of a plurality of series of hydrolyzed polymer fragments, and optionally provide statistical interpretation paradigms and computer software therefor. The apparatus involves a mass spectrometer sample holder, having hydrolyzing agent disposed thereon, which is useful for adapting any mass spectrometer for polymer sequencing.

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