Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic
Reexamination Certificate
2008-12-11
2011-12-06
Yang, N (Department: 1641)
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is inorganic
C435S007100, C435S286500, C435S289100, C436S518000, C436S536000
Reexamination Certificate
active
08071395
ABSTRACT:
Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.
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Davis Ronald W.
Hajhossein Talasaz AmirAli
Jeffrey Stefanie S.
Mindrinos Michael N.
Pease R. Fabian
Aston David J.
Peters Verny LLP
The Board of Trustees of the Leland Stanford Junior University
Yang N
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