Methods and apparatus for low resistance electrophoresis of...

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

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C204S456000, C204S606000, C204S616000

Reexamination Certificate

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08034223

ABSTRACT:
Methods and apparatus are presented that facilitate electrophoresis of prior-cast, hydratable separation media, usefully immobilized pH gradient (IPG) strips. The method exploits the swelling of prior-cast, hydratable separation media upon rehydration to help lodge the media in an enclosing member that permits spaced electrical communication with the enclosed separation media. The electrical communication permits a voltage gradient to be established in the enclosed separation medium sufficient to effect separation of analytes therein. Cassettes, buffer cores, electrophoresis systems and kits are presented for effecting the methods of the invention.

REFERENCES:
patent: 3875044 (1975-04-01), Renn et al.
patent: 4094759 (1978-06-01), Ruhenstroth-Bauer et al.
patent: 4130470 (1978-12-01), Rosengren et al.
patent: 4374723 (1983-02-01), Vesterberg
patent: 4385974 (1983-05-01), Shevitz
patent: 4415428 (1983-11-01), Nochumson et al.
patent: 4417967 (1983-11-01), Ledley
patent: 4443319 (1984-04-01), Chait et al.
patent: 4666581 (1987-05-01), Itoh et al.
patent: 4693804 (1987-09-01), Serwer
patent: 4746551 (1988-05-01), Allen et al.
patent: 5149418 (1992-09-01), Flesher
patent: 5159049 (1992-10-01), Allen
patent: 5209831 (1993-05-01), MacConnell
patent: 5238651 (1993-08-01), Chuba
patent: 5275710 (1994-01-01), Gombocz et al.
patent: 5389400 (1995-02-01), Ting et al.
patent: 5407546 (1995-04-01), Schickle
patent: 5543023 (1996-08-01), Lugojan
patent: 5593838 (1997-01-01), Zanzucchi et al.
patent: 5699157 (1997-12-01), Parce
patent: 5707506 (1998-01-01), Douthart et al.
patent: 5744336 (1998-04-01), Hodges et al.
patent: 5746901 (1998-05-01), Balch et al.
patent: 5773645 (1998-06-01), Hochstrasser
patent: 5800690 (1998-09-01), Chow et al.
patent: 5827418 (1998-10-01), Haven et al.
patent: 5837116 (1998-11-01), Harrington et al.
patent: 5888369 (1999-03-01), Tippins et al.
patent: 5989400 (1999-11-01), Islam
patent: 5993627 (1999-11-01), Anderson et al.
patent: 6001233 (1999-12-01), Levy
patent: 6013165 (2000-01-01), Wiktorowicz et al.
patent: 6113766 (2000-09-01), Steiner et al.
patent: 6156182 (2000-12-01), Olech et al.
patent: 6398933 (2002-06-01), Scott
patent: 6558522 (2003-05-01), Williams et al.
patent: 6936150 (2005-08-01), Rooney et al.
patent: 7601251 (2009-10-01), Rooney et al.
patent: 2003/0015426 (2003-01-01), Rooney et al.
patent: 19930253 (2000-12-01), None
patent: 1385603 (2004-02-01), None
patent: 62-167460 (1987-07-01), None
patent: 4236938 (1992-08-01), None
patent: WO-94/10561 (1994-05-01), None
patent: WO-98/45693 (1998-10-01), None
patent: WO-98/57161 (1998-12-01), None
patent: WO-98/57162 (1998-12-01), None
patent: WO-99/33550 (1999-07-01), None
patent: WO-00/31526 (2000-06-01), None
patent: WO-01/20315 (2001-03-01), None
patent: WO-02/26773 (2002-04-01), None
patent: WO-02/92200 (2002-11-01), None
U.S. Appl. No. 09/633,172, filed Aug. 2000, Champagne, James T.
U.S. Appl. No. 09/633,172; Notice of Allowance mailed Nov. 28, 2008.
U.S. Appl. No. 10/102,188; Notice of Allowance mailed Mar. 24, 2005.
U.S. Appl. No. 10/102,188; Office Action mailed Sep. 27, 2004.
U.S. Appl. No. 10/102,188; Office Action mailed Dec. 22, 2003.
U.S. Appl. No. 10/102,188; Response to Sep. 27, 2004 Office Action mailed Jan. 27, 2005.
U.S. Appl. No. 10/102,188; Response to Dec. 22, 2003 Office Action mailed May 24, 2004.
U.S. Appl. No. 10/464,258; Notice of Allowance mailed Apr. 14, 2009.
U.S. Appl. No. 10/464,258; Office Action mailed Sep. 18, 2007.
U.S. 10/464,258; Non-Final Office Action mailed Jan. 25, 2007.
U.S. Appl. No. 10/464,258; Response to Final Office Action mailed Sep. 18, 2008.
U.S. Appl. No. 10/464,258; Response to Non-Final Office Action mailed Jun. 25, 2007.
EP02713876; Supplementary EPO Search Report mailed Nov. 26, 2004.
EP02713876; European Search report mailed Sep. 10, 2004.
PCT/US02/08438; PCT International Search Report mailed Sep. 5, 2002.
WO 2003/106973; PCT Search Report mailed Dec. 29, 2003.
Amersham Pharmacia Biotech, “-2-D Electrophoresis Using Immobilized pH Gradients: Principles and Methods”,Rev. A, part 80-6429-60, (Sep. 1998); pp. 1-51.
Amersham Pharmacia Biotech, “Immobiline Dry Strip”, 1998; pp. 1-4.
Amersham Pharmacia Biotech, “IPGphor IEF System”, www.apbiotech.com/stiboasp/showmodule.asp? (Feb. 23, 2001); pp. 1-7.
Bio-Rad Laboratories, “2-D Electrophoresis: ReadyStrip IPG Strips: Part of the ProteomeWorks System”, http://www.expressionproteomics.com/LifeScience/pdf/Bulletin—2442.pdf Bulletin 2442 Rev F; pp. 1-2.
Bio-Rad Laboratories, “A Flexible, High Throughput Method for 2-D Protein Separations”, EG Bulletin 2217; pp. 1-2.
Bio-Rad Laboratories, “Isoelectric Focusing Cell : Model 111 Mini IEF Cell”,Bio-Rad Laboratories(Life Science Research catalog) Website Apr. 6, 1997., www.biorad.com/56537.html Apr. 6, 2007; 2 pages.
Bio-Rad Laboratories, “Protean II xi Cell and Protean II xi 2-D Cell Instruction Manual”; pp. 1-49.
Bio-Rad Laboratories, “Protean II xi and XL Multi-Cells:Ordering Information”, www.bio-rad.com/B2B/BioRad/Product/br—category.jsp?BV. Original url no longer available. Attachment uploaded Aug. 11, 2008 Feb. 27, 2002, 1-2.
Bio-Rad Laboratories, “Protean II xi Cell IPG Conversion Kit Setup Guide”, Feb. 23, 2001; pp. 1-2.
Bio-Rad Laboratories, “Protean II xi multi-cell Instruction Manual Catalog No. 165-1951”; pp. 1-23.
Bio-Rad Laboratories, “Protean II XL Cell for IPG Strips”, www.bio-rad.com/B2B/BioRad/product/br—category.jsp?BV Feb. 23, 2002; 1 page.
Bio-Rad Laboratories, “Protein Electrophoresis: Large Precast Gels for 1-D, Protean II Ready Gel Precast Gels for Single-Dimension Gel Electrophoresis”.
Bio-Rad Laboratories, “Protein Electrophoresis: Large Precast Gels for 2-D: Part of the ProteomeWorks System, Ready Gel Precast Gels for Two-Dimensional Gel Electrophoresis”.
Bio-Rad Laboratories, “ReadyStrip.TM. IPG Strips Instruction Manual”, Catalog No. 163-2099; pp. 1-11.
Bio-Rad Laboratories, “The ProteomeWorks System: Now Get More From 2-D”.
Bjellqvist, Bengt, “Isoelectric focusing in immobilized pH gradients: principle, methodology and some applications”,Journal of Biochemical and Biophysical Methods, vol. 6, No. 4, Elsevier Biomedical Press 1982; pp. 317-339.
Bonnet, “Epoxy-Diamine Thermoset/Thermoplastic Blends.1. Rates of Reaction before and after Phase Separation”,Macromolecules, vol. 32, No. 25, 1999; pp. 8517-8523.
Bonnet, “Epoxy-Diamine Thermoset/Thermoplastic Blends.2. Rheological Behavior before and after Phase Separation”,Macromolecules, vol. 32, No. 25, 1999; pp. 8524-8530.
Bonnet, “Epoxy-Diamine Thermoset/Thermoplastic Blends: Dielectric Properties before, during, and after Phase Separation”,Macromolecules, vol. 33, No. 10, 2000; pp. 3833-3843.
Frey, Manuela D., “Preparation of Rehydratable Polyacrylamide gels and Their Application in Ultrathin-Layer Isoelectric Focusing”,Electrophoresis, vol. 7, 1986; pp. 28-40.
Gorg, Angelika, “The Current State of Two-Dimensional Electrophoresis with Immobilized pH Gradients”,Electrophoresis, vol. 21, No. 6, 2000; pp. 1037-1053.
Gorg, Angelika, “The Current State of Two-Dimensional Electrophoresis with Immobilized pH Gradients”,Electrophoresis, vol. 9, No. 9, 1988; pp. 531-546.
Haglund, Herman, “Isoelectric Focusing in pH Gradients-A Technique for Fractionalization and Characterization of Ampholytes”,Methods of Biochemical Analysis, vol. 19, Interscience Publishers, Edited by David Glick 1971; pp. 1-104.
Hanash, Samir M., “Two-dimensional electrophoresis with immobilized pH gradients in the first dimension: Protein focusing as a function of time”,Electrophoresis, vol. 8, 1987; pp. 229-234.
Harrington, M.G., “Analytical and Micropreparative Two-Dimensional Electrophoresis of Proteins”,Methods: a Companion to Methods in Enzymolo

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