Chemistry: physical processes – Physical processes – Crystallization
Patent
1978-03-06
1980-11-11
Scovronek, Joseph
Chemistry: physical processes
Physical processes
Crystallization
23230B, 73 611C, 250437, 250461R, 356 72, 422 70, 422186, G01N 3108, G01N 3316, G01N 2138, G01J 110
Patent
active
042330300
ABSTRACT:
The eluent from a liquid chromatography column is irradiated, while flowing between the column and a conventional detector adapted to detect a given property, in order to convert a known or suspected constituent photochemically to a species which possesses the property or to one in which the property is changed in magnitude (enhanced or reduced) to a substantial degree. The irradiation may be by UV or visible light, and the property may eg be fluorescence with, or absorbance of, UV or visible light. The method can increase the sensitivity and/or selectivity of detection, eg in the presence of interfering substances which do not react to irradiation in the same way as the constituent of interest.
Apparatus for performing the method may comprise a long translucent small-bore tube shaped to a configuration which surrounds a light-source and which is connected between the column and the detector.
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Bowd et al., Ultraviolet Absorption and Luminescence Properties of Some Cannabis Constituents, Talanta, vol. 18, No. 7, pp. 697-705 (Jul., 1971).
Wu et al., Analysis of Purine and Strychnos Alkaloids by High-Speed Liquid Chromatography, Analytical Chem., vol. 44, No. 8, pp. 1499-1501 (Jul., 1972).
Twitchett Peter J.
Williams Peter L.
National Research Development Corporation
Scovronek Joseph
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