Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-10-15
1996-07-30
Spiegel, Carol A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 7200, 435 7230, 436 10, 436 63, 436 64, 422 68100, 356 39, 356318, 356320, 364413080, 364413100, 382129, 382133, 382134, C12M 300
Patent
active
055410642
ABSTRACT:
A method and apparatus are provided for selecting and analyzing a subpopulation of cells or cell objects for a certain parameter such as DNA using image analysis means. The cells are first stained with an alkaline phosphatase technique including a monoclonal antibody specific to a protein in at least one of the cell's cytoplasm or on a cell membrane, thereby marking any cells including the protein as to type. A second staining of the DNA in the nucleus is accomplished by a Feulgen technique that destroys the cell cytoplasm. After the staining and marking, the cells may then be gated using the image analysis means on the visual parameter such as colored DNA or colored antigen into a subpopulation that is to be measured. The selected cells may then be examined by digital image processing and measured for a parameter such as a true actual measurement of DNA in picograms. A quantitation of the measured parameter may be generated and provided.
REFERENCES:
patent: 4998284 (1991-03-01), Bacus et al.
patent: 5134662 (1992-07-01), Bacus et al.
R. Riley, "Cellular Proliferation Markers In the Evaluation of Human Cancer," Clinics In Laboratory Medicine, vol. 12, No. 2 (1992) 163-199.
B. Schwartz et al, "Cell Proliferation in Non-Hodgkin's Lymphoms Digital Image Analysis of Ki67 Antibody Staining", Am. J. Pathol vol. 134, No. 2 (1989) 327-336.
Bacus James W.
Marder Robert J.
Cell Analysis Systems, Inc.
Spiegel Carol A.
Wark Allen W.
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