Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1994-03-18
1995-06-27
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 32, 435291, 435808, 356 39, 356339, 356436, 356342, C12Q 104, C12M 134, G01N 3348, G01N 2100
Patent
active
054279208
ABSTRACT:
Methods and apparatus for detecting biological activities in a specimen such as blood are disclosed. The present invention provides a system whereby the intensity of light with a wavelength within the range of about 600 nm to 800 nm introduced into a sample at a first location is measured as it reemerges at a second location. A significant change in the intensity of the reemerging light indicates the presence of biological activity. Preferably, the intensity is also measured at a fourth location, or alternatively, a second light source is disposed at a third location to permit comparative intensity data to be collected. These data are useful in partially identifying the types of microorganisms present in the sample. In preferred embodiments, light emitting diodes are used as the light sources and multiplexed to a plurality of samples. These signals are either detected using photodetectors and demultiplexed, or are collected using fiber-optic light guides and fed to a photomultiplier tube which generates an intensity signal. In either embodiment, the intensity signal is preferably amplified, digitized and stored in a computer.
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Berger Dolores M.
Berndt Klaus W.
Gladnick Paul G.
Becton Dickinson and Company
Fiedler Alan W.
Snider Theresa T.
Warden Robert J.
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