Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Patent
1995-04-05
2000-05-30
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
435468, 435471, 435483, 435 698, 435455, 536 232, 536 236, 800281, 800300, C12N 1529, C12N 1552, C12N 1570, C12N 1574, C12N 1581, C12N 1582
Patent
active
060692987
ABSTRACT:
The present invention provides the complete cDNA sequence of maize acetyl CoA carboxylase and methods for conferring herbicide tolerance and/or altering the oil content of plants by introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. The method of imparting herbicide tolerance to a plant includes the steps of introducing an expression cassette encoding a plant acetyl CoA carboxylase or an antisense DNA sequence complementary to the sequence for a plant acetyl CoA carboxylase gene operably linked to a promoter functional in plant cells, into the cells of a plant tissue and expressing the plant acetyl CoA carboxylase gene in an amount effective to render the acetyl CoA carboxylase and/or plant cell substantially tolerant to the herbicides. The method of altering the oil content in a plant includes the steps of introducing an expression cassette into plant cells and expressing the acetyl CoA carboxylase gene in an amount effective to alter the oil content of the cells. The expression cassette can also be introduced into other host cells to increase yield of a plant acetyl CoA carboxylase so that crystallized enzyme can be used to screen and identify other herbicides that bind to and inhibit the enzyme.
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Egli Margaret A.
Gengenbach Burle G.
Gronwald John W.
Lutz Sheila M.
Somers David A.
Fox David T.
Nelson Amy J.
Regents of the University of Minnesota
The United States of America
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