Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Reexamination Certificate
2005-09-06
2005-09-06
Le, Long V. (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
C435S007800, C436S173000, C422S051000, C356S301000
Reexamination Certificate
active
06939686
ABSTRACT:
A method of using Raman imaging microscopy to evaluate drug actions in living cells is disclosed. Specifically the invention describes the methods of using Raman imaging microscopy to detect drug uptake, distribution, binding, and metabolism in a single cell, and to study drug pharmacokinetics at the cellular level. The method involves measuring the Raman image of both the drug and the cell. Control images and post-treatment images of the cell were studied. Ratio images were calculated and the requisite information was obtained from a study of the intensity of the bright areas in the ratio images.
REFERENCES:
patent: 4405237 (1983-09-01), Manuccia et al.
patent: 4847240 (1989-07-01), Ryser et al.
patent: 5580750 (1996-12-01), Doglia et al.
patent: 5841139 (1998-11-01), Sostek et al.
patent: 5902732 (1999-05-01), Hochman
patent: 5965352 (1999-10-01), Stoughton et al.
patent: 6002471 (1999-12-01), Quake
patent: 6002476 (1999-12-01), Treado
Beljebbar et al, Raman and SERS microspectroscopy on living cells: a promising tool toward cellular drug response and medical diagnosis, Proceedings of SPEI-The Internationsl Society for Optical Engineering (1999), vol. 3608, (Biomedical Applications on Continued: Raman Spectroscopy), pp. 175-184.
Whitley et al, Prodeedings of SPIE-The International Society for Optical Engineering (1998) vol. 3261, (Three-Dimensional Microscopy: Image Acquistion and Processing V), pp. 250-259.
Otto et al, Applicantions iof micro-Raman imaging in biomedical research, Journal of Raman Spectroscopy (1997), vol. 28 (2 and 3), pp. 143-150.
Paloley, J., The Handbook of Biological Confocal Microscopy, JMR Press 1989.
Manoharan R., Wang Y., Feld MS, “Histochemical Analysis of Biological Tissue Using Raman Spectroscopy”,Spectrochimica Acta, Part A, 52, pp 210-249, 1996.
Ferraro Jr, et al, Introductory Raman Spectroscopy, Academic Press, Jul. 1994.
Brenan CJH, et al, “Volumetric Raman Spectral Imagaging with a Confocal Raman Microscope: Image Modalities & Applications” SPIE v 26ss, pp 130-139, 1996.
Kincade K, Raman Spectroscopy Enhances in vivo Diagnosis Laser Focus World, pp 83-91, Jul. 1998.
Turrell G, et al, Raman Microscopy-Developments & Applications, Academic Press 1996.
Buick RN, “Cellular Basis of Chemotherapy”, Cancer Chemotherapy Handbook, Appletin & Lange Press 1994.
Jones RA, An Automated Technique for Deriving MTF from Edge Traces Photographic Science & Engineering VII (2), pp 102-106, 1967.
Blackman ES, Effects of Noise on the Determination of Photographic System Modulation Transfer Functions. Photographic Science & Engineering V12(5) pp 244-250, 1968.
Bovik, AC, “On Detecting Edges in Speckle Imagery”, IEEE Trans. Acoust., Speech, Signal Processing, V36(10), pp 1618-1627, 1988.
Kondo K, et al, “Image Restoration by Weiner Filtering in the Presence of Signal-Dependent Noise”, Appl. Opt., V16(0), pp 2254-2558, 1977.
Lim JS, Two-Dimensional Signal & Image Processing, Englewood Cliffs, Prentice Hall, pp 536-540, 1990.
Castleman KR, Digital Image Processing, Prentice Hall, Englewood Cliffs, New Jersey, pp 117, 1996.
Art J., “Photon Dectectors for Confocal Microscopy”, Handbook of Biological Confocal Microscopy, Plenum Press, New York, pp 183-196, 1995.
Lee JS, Digital Image Enhancement and Noise Filtering by Use of Local Statistics, IEEE Trans. Pattern Anal. Mach. Intell, PAM 1-2, pp 165-168, 1980.
Perona P, Scale Space & Edge Detection Using Anistrophic Diffusion, IEE Trans. Pattern Anal. Mach. Intell, vol 12(7) pp 629-639, 1990.
Sijtsema, Nmetal, Confocal Direct Imaging Raman Microscope: Design and Applications in Biology, Applied Spechoscopy, v52(3), pp 348-355, 1998.
Freeman TL, et al, Investigation of the Subcellular Localization of Zinc Phthalocyanines by Raman Mapping, Applied Spectroscopy, v52(10), pp 1257-1263, 1998.
S. Sharonov, I. Chourpa, H. Morjani, IL Nabiev and M. Manfait; Confocal spectral imaging analysis in studies of the spatial distribution of anitumour drugs within living cancer cells.
G.D. Sockalingum, A. Beljebbar, H. Morjani, J.F. Angiboust, M. Manfait; Characterization of Island Films as Surface-Enhanced Raman Spectroscopy Substrates for Detecting Low Antitumor Drug Concentrations at Single Cell Level.
Ling Jian
Miller Michael A.
Weitman Steven D.
Davis Deborah A.
Gunn & Lee PC
Le Long V.
Southwest Research Institute
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