Methodology for colorimetrically determining the concentration o

Details Methodology for colorimetrically determining the concentration o Methodology for colorimetrically determining the concentration o

1996-02-26

1997-09-02

Kight, John

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 724, 435 18, 435 24, 435 19, 435 23, 435963, 435970, 4352831, 4352871, C12Q 100, C12Q 134, C12Q 137, G01N 3353

Patent

active

056630441

ABSTRACT:
A composition, method and test device for determining the presence of leukocytes, esterase or protease in a test sample are disclosed. The composition comprises an ester which is hydrolyzed in the presence of leukocyte, esterase, or protease to form a reaction product which couples with a diazonium salt to produce a detectable color change. The composition further comprises the salt of an alkaline earth metal which stabilizes the composition during manufacture and prevents the occurrence of false or background color change due to reactivity of the diazonium salt in the absence of leukocyte, esterase or protease.

REFERENCES:
patent: 4554236 (1985-11-01), Bentley et al.
Kutter et al, "J. Clin. Chem. Clin. Biochem", vol. 25, 1987, pp. 91-94. (Month of Publication Not Available; Please Print).
Benoist et al, "Analytical Biochemistry", vol. 187, pp. 337-344, 1990. (Month of Publication Not Available; Please Print).
Brown et al, "J. of Chromatography", p. 169 (1979) pp. 407-408. (Month of Publication Not Available).
Miller et al, "J. of Biochem & Biophysical Meth", 3 (1980) pp. 345-354.
Bardi et al, "J. Inst. Brew.", vol. 99, pp. 385-388, Oct. 1993.

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