Method, vectors, and host cells for the control of expression of

Details Method, vectors, and host cells for the control of expression of Method, vectors, and host cells for the control of expression of

1989-07-27

1992-12-08

Lacey, David L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352523, 4353201, 536 27, C12N 1511, C12N 1567

Patent

active

051697602

ABSTRACT:
The invention is a process for controlling the expression of heterologous genes from lac-operated promoters by removing the CAP binding site and lac promoter from the lac operon. Illustrated is the fusion of the lacZ, Y, and A genes of the lac operon to the 3' end of the lacI structural gene. This elimination of the natural regulatory elements of the lac operon results, advantageously, in the production of lac operon gene products in a constitutive mode from the lacI promoter.

REFERENCES:
Gene, 51:255-267, 1987, Stark, Multicopy Expression Vectors Carrying the lac Repressor Gene for Regulated High-Level Expression of Genes in Escherichia coli.
Beckwith, J., (1987), "The Lactose Operon", in Escherichia coli and Salmonella typhimurium Cellular and Molecular Biology, vol. 2, Neidhardt, F. C., Editor in Chief, American Society for Microbiology, Washington, D.C.
Freifelder, D., (1987), "Molecular Biology", Jones and Bartlett Publishers, Inc., Portola Valley, Calif.
Jobe, A., Bourgeois, S., (1972), "The Natural Inducer of the lac Operon", J. Mol. Biol., 69:397-408.
Muller-Hill, H. V. Rickenberger and K. Wallenfels, (1964), "Specifiecity of the Induction of the Enzymes of the lad Operon in Escherichia coli", J. Mol. Biol., 10:303-318.

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