Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...
Patent
1994-09-16
1995-12-19
Knode, Marian C.
Chemistry: molecular biology and microbiology
Maintaining blood or sperm in a physiologically active state...
424533, A01N 102
Patent
active
054767644
ABSTRACT:
Method using CO for extending the useful shelf-life of refrigerated red blood cells. Carbon monoxide is utilized for stabilizing hemoglobin in red blood cells to be stored at low temperature. Changes observed in the stored cells are similar to those found in normal red cell aging in the body, the extent thereof being directly related to the duration of refrigerated storage. Changes in cell buoyant density, vesiculation, and the tendency of stored cells to bind autologous IgG antibody directed against polymerized band 3 IgG, all of which are related to red blood cell senescence and increase with refrigerated storage time, have been substantially slowed when red blood cells are treated with CO. Removal of the carbon monoxide from the red blood cells is readily and efficiently accomplished by photolysis in the presence of oxygen so that the stored red blood cells may be safely transfused into a recipient.
REFERENCES:
patent: 5189146 (1993-02-01), Hsia
Valeri C. R. in Blood Banking and the Use of Frozen Blood Products, CRC Press pp. 35-39 (1976).
Richard E. Waugh et al., "Rheologic Properties Of Senescent Erythrocytes: Loss Of Surface Area And Volume With Red Blood Cell Age," Blood 79, 1351 (1992).
Ken Ando et al., "Increased Susceptibiity Of Stored Erythrocytes To Anti-Band 3 IgG Autoantibody Binding," Biochimica et Biophysica Acta 1178, 127 (1993).
Phillip S. Low et al., "The Role Of Hemoglobin Denaturation And Band 3 Clustering In Red Blood Cell Aging," Science 227, 531 (1985).
Charles E. Huggins, "Frozen Blood; Principles of Practical Preservation," Monographs in the Surgical Sciences, 3, 133 (1966).
C. R. Valeri et al., "The Safety And Therapeutic Effectiveness Of Human Red Cells Stored At -80.degree. C. For As Long As 21 Years," Transfusion 29, 429 (1989).
R. Blaine Moore et al., "Ascorbate Protects Against Tert-Butyl Hydroperoxide Inhibition Of Erythrocyte Membrane Ca.sup.2+ -+Mg.sup.2+ ATPase," Arch. Biochem. and Biophys. 278, 416 (1990).
Tanya Repka et al., "Hydroxyl Radical Formation By Sickle Erythrocyte Membranes: Role Of Pathologic Iron Deposits And Cytoplasmic Reducing Agents," Am. Soc. Hematology 10, 2753 (1991).
Eraldo Antonini et al., "Hemoglobin And Myoglobin In Their Reactions With Ligands," Frontiers of Biology, vol. 21, North-Holland Publishing Company, Amsterdam-London (1971).
J. McKenney et al., "Decreased In Vitro Survival Of Hydrogen Peroxide-Damaged Baboon Red Blood Cells," Blood 76, 206 (1990).
Freund Samuel M.
Knode Marian C.
Saucier S.
The Regents of the University of California
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