Method using azide catalyst for peroxyoxalate chemiluminescence

Details Method using azide catalyst for peroxyoxalate chemiluminescence Method using azide catalyst for peroxyoxalate chemiluminescence

1994-06-13

1996-02-27

Snay, Jeffrey R.

Chemistry: analytical and immunological testing

Optical result

With fluorescence or luminescence

436135, G01N 2176

Patent

active

054948274

ABSTRACT:
An azide catalyst for peroxyoxalate chemiluminescence reactions is provided, which catalyzes a reaction of an oxalic acid ester compound with a peroxide in a solvent to produce 1,4 dioxetane dione as an intermediate that is subsequently decomposed catalytically by a fluorescent agent to emit light. The catalyst improves not only luminous efficiency such as luminous time and luminous intensity but also reaction of the intermediate with a peroxide.

REFERENCES:
patent: 4647532 (1987-03-01), Watanabe et al.
patent: 5225584 (1993-07-01), Brooks et al.
Imai, K. "Chemiluminescence Detection System for High-Performance Liquid Chromatography" Methods in Enzymology, vol. 133 (1986) pp. 435-449.
Seitz, W. R. "Chemiluminescence Detection of Enzymically Generated Peroxide" Methods in Enzymology, vol. 57 (1978) pp. 445-462.
Sang Kon Oh and Seung Hee Cha, Analytical Biochemistry 218:222-4, 1994.

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