Method, use and kit for separating albumin from contaminants...

Details Method, use and kit for separating albumin from contaminants... Method, use and kit for separating albumin from contaminants...

2008-04-01

2008-04-01

Mondesi, Robert (Department: 1656)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Albumin

C530S413000

Reexamination Certificate

active

07351801

ABSTRACT:
The present invention is a method of separating recombinant human serum albumin (rHSA) from low molecular weight contaminants in a liquid, which method comprises the steps of: (a) providing a separation medium, which includes anion-exchanging ligands coupled to a base matrix; and (b) contacting the liquid with the separation medium to adsorb the rHSA to the ligands. In one embodiment, the functional groups of the ligands are weak anion-exchanging groups, preferably secondary amines, and the density of ligands on the base matrix is relatively high.

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Quirk et al., “Production of recombinant human serum albumin fromSaccharomyces cerevisiae”, Biotechnol. Appl. Biochem. 11: 273-287 (1989).

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