Method to transform algae, materials therefor, and products prod

Details Method to transform algae, materials therefor, and products prod Method to transform algae, materials therefor, and products prod

1995-03-15

1997-08-26

Fox, David T.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, 435 701, 435 711, 4352572, 4353201, 435 6, 435420, 536 236, 536 237, 536 241, 800205, A01H 1300, C12N 113, C12N 1529, C12N 1531, C12N 1579

Patent

active

056610173

ABSTRACT:
Disclosed is a method to transform chlorophyll C-containing algae which includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further specifically disclosed are molecules pACCNPT10, pACCNPT4.8 and pACCNPT5.1. The methods and materials of the present invention provide the ability to accomplish stable genetic transformation of chlorophyll C-containing algae.

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