Method to purify basic fibroblast growth factor

Details Method to purify basic fibroblast growth factor Method to purify basic fibroblast growth factor

1990-04-04

1992-08-04

Cashion, Jr., Merrell C.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530350, 530397, 530399, 530412, 530415, 530416, 530412, C07K 300, C07K 312, C07K 318

Patent

active

051360250

ABSTRACT:
This invention relates to a method of isolating and purifying proteins such as fibroblast growth factor using metal chelate affinity column chromatography and without the use of heparin. Also disclosed is a method of recovering bFGF multimers and purifying bFGF using metal chelate affinity column chromatography in the absence of heparin.

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Weselake et al., Analyt. Biochem. (1986) 155:193-197.
Sulkowski, Protein Purification: Micro to Macro (1987), Alan R. Liss, Inc., pp. 149-162.

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