Method to produce sterile male flowers and partenocarpic...

Details Method to produce sterile male flowers and partenocarpic... Method to produce sterile male flowers and partenocarpic...

2011-08-09

2011-08-09

Baum, Stuart F. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide encodes an inhibitory rna molecule

C800S287000, C536S024500

Reexamination Certificate

active

07994397

ABSTRACT:
Genes VvPI fromVitis viniferacv. Cabernet Sauvignon and LePI fromLycopersicon esculentumare described; together with the use of these genes to produce sterile male flowers and seedless or parthenocarpic fruits. Silencing vectors that comprise these sequences or a part thereof are disclosed. The methods of the invention are directed to producing sterile male flowers and parthenocarpic fruits by genetic silencing, and includes: obtaining the codifying sequence of Pistillata (PI)-homologous genes from the target species; analyzing the expression of the sequence obtained in step (a) to test its expression according to the pattern described for Pistillata genes; analyzing the complementation of PI-gene mutant with the PI sequence obtained from the target species, to assess that the obtained sequence fulfills the function of a PI gene; making a genetic silencing construct that comprises a region of the codifying sequence of PI in a plant expression vector; incorporation of the constructed vector intoAgrobacterium tumefaciens; transforming target plants withAgrobacterium tumefaciensmodified with the silencing vector and selecting said transformed plants; and checking the absence ofAgrobacteriumcontamination and corroborating transgenic plants by transgene amplification.

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