Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1997-07-17
2000-05-23
MacMillan, Keith D.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 232, 4353201, 43525235, 4352523, 435 71, C12N 1511, C12N 1563, C12N 120
Patent
active
060667214
ABSTRACT:
Modified PKS gene clusters which produce novel polyketides in an efficient system in a host cell or in a cell free extract are described.
REFERENCES:
patent: 5475099 (1995-12-01), Knauf et al.
patent: 5824513 (1998-10-01), Katz
McDaniel, R., et al., "Gain-of-Function Mutagenesis of a Modular Polyketide Synthase," J Am Chem Soc (1997) 119(18):4309-4310.
Kramer, P.J., et al., "Rational Design and Engineered Biosynthesis of a Novel 18-Carbon Aromatic Polyketide," J Am Chem Soc (1997) 119(4):635-639.
Kao, C.M., et al., "Gain of Function Mutagenesis of the Erythromycin Polyketide Synthase. 2. Engineered Biosynthesis of an Eight-Membered Ring Tetraketide Lactone," J Am Chem Soc (1997) 119(46):11339-11340.
Wiesmann, K.E.H., et al., "Polyketide synthesis in vitro on a modular polyketide synthase," Chemistry & Biology (1995) 2(9):583-589.
Kao, C.M., et al., "Manipulation of Macrolide Ring Size by Directed Mutagenesis of a Modular Polyketide Synthase," J Am Chem Soc (1995) 117(35):9105-9106.
Kao, C.M., et al., "Engineered Biosynthesis of Structurally Diverse Tetraketides by a Trimodular Polyketide Synthase," J Am Chem Soc (1996) 118(38):9184-9185.
Donadio, S., et al., "Modular Organization of Genes Required for Complex Polyketide Biosynthesis," Science (1991) 252:675-679.
Donadio, S., et al., "An erythromycin analog produced by reprogramming of polyketide synthesis," Proc Natl Acad Sci USA (1993) 90:7119-7123.
Bedford, D., et al., "A functional chimeric modular polyketide synthase generated via domain replacement," Chemistry & Biology (1996) 3(10):827-831.
Oliynyk, M., et al., "A hybrid modular polyketide synthase obtained by domain swapping," Chemistry & Biology (1996) 3(10):833-839.
Kuhstoss, S., et al., "Production of a novel polyketide through the construction of a hybrid polyketide synthase," Gene (1996) 183:231-236.
Omura, et al., "Inhibition of the Biosynthesis of Leucomycin, a Macrolide Antibiotic, by Cerulenin," J. Biochem. 75:193-195 (1974).
Rudd, et al., "Genetics of Actinorhodin Biosynthesis by Streptomyces Coelicolor A3(2)," J. Gen. Microbiol. 114:35-43 (1979).
Donadio S, et al, (1992) Biosynthesis of the erythromycin macrolactone and a rational approach for producing hybrid macrolides. Gene 115: 97-103, 1992.
Cane DE, et al, (1993) Macrolide biosynthesis. 7. Incorporation of polyketide chain elongation intermediates into methymycin. J.Am.Chem.Soc. 115:522-526, 1993.
Kao CM, et al, (1994) Engineered biosynthesis of a complete macrolactone in a heterologous host. Science 265:509-512, 1994.
Aparicio et al., "Limited Proteolysis and Active-Site Studies of the First Multienzyme Component of the Erythromycin-Producing Polyketide Synthase," J. of Biol. Chem. 269(11):8524-8528 (1994).
Bartel et al., "Biosynthesis of Anthraquinones by Interspecies Cloning of Actinorhodin Biosynthesis Genes in Streptomycetes: Clarification of Actinorhodin Gene Functions," J. Bacteriol. 172(9):4816-4826 (1990).
Beck et al., "The Multifunctional 6-methylsalicylic Acid Synthase Gene of Penicillium Patulum. Its Gene Structure Relative to that of Other Polyketide Synthases," Eur. J. Biochem. 192:487-498 (1990).
Bevitt et al., "6-Deoxyerythronolide-B Synthase 2 from Saccharopolyspora erythraea: Cloning of the Structural Gene, Sequence Analysis and Inferred Domain Structure of the Multifunctional Enzyme," Eur. J. Biochem. 204:39-49 (1992).
Bibb et al., "Analysis of the Nucleotide Sequence of the Streptomyces Glaucescens tcml Genes Provides Key Information about the Enzymology of Polyketide Antibiotic Biosynthesis," EMBO J. 8(9):2727-2736 (1989).
Caballero et al., "Organisation and Functions of the actVA Region of the Actinorhodin Biosynthetic Gene Cluster of Streptomyces coelicolor," Mol. Gen. Genet. 230:401-412 (1991).
Caffrey et al., "An Acyl-Carrier-Protein--Thioesterase Domain from the 6-Deoxyerythronolide B Synthase of Saccharopolyspora erythraea. High-Level Production, Purification and Characterisation in Escherichia coli," Eur. J. Biochem. 195:823-830 (1991).
Caffrey et al., "Identification of DEBS 1, DEBS 2 and DEBS 3, the Multienzyme Polypeptides of the Erythromycin-Producing Polyketide Synthase from Saccharopolyspora erythraea," FEBS Lett. 304(2):225-228 (1992).
Corcoran et al., "The Biogenesis of Fatty Acids and Erythronolide-Like Substances in Mycelium-Free Extracts of Streptomyces Erythreus," in 5th International Congress of Chemotherapy, Vienna, Abstracts of Communications, pp. 35-40 (1967).
Corcoran, ed., in Antibiotics Volume IV Biosynthesis, Springer-Verlag, New York, pp. 145-150 (1982).
Cortes et al., "An Unusually Large Multifunctional Polypeptide in the Erythromycin-Producing Polyketide Synthase of Saccharopolyspora erythraea," Nature 348:176-178 (1990).
Davis et al., "Functional Mapping of a Polyketide Synthase from aspergillus terreus involved in Lovastatin Synthesis," Abst. of the Genetics of Industrial Microorganisms Mtg. P288:192 (1994).
Dimroth et al., "Biosynthese von 6-Methylsalicylsaure," Eur. J. Biochem. 13:98-110 (1970).
Donadio et al., "Modular Organization of Genes Required for Complex Polyketide Biosynthesis," Science 252:675-679 (1991).
Donadio et al., "Organization of the Enzymatic Domains in the Multifunctional Polyketide Synthase Involved in Erythromycin Formation in Saccharopolyspora erythraea," Gene 111:51-60 (1992).
Fernandez-Moreno et al., "The act Cluster Contains Regulatory and Antibiotic Export Genes, Direct Targets for Translational Control by the bldA tRNA Gene of Streptomyces," Cell 66:769-780 (1991).
Fernandez-Moreno et al., "Nucleotide Sequence and Deduced Functions of a Set of Cotranscribed Genes of Streptomyces coelicolor A3(2) Including the Polyketide Synthase for the Antibiotic Actinorhodin," J. Biol. Chem. 267:19278-19290 (1992).
Hallam et al., "Nucleotide Sequence, Transcription and Deduced Function of a Gene Involved in Polyketide Antibiotic Synthesis in Streptomyces coelicolor," Gene 74:305-320 (1988).
Hopwood et al., "Product of `Hybrid` Antibiotics by Genetic Engineering," Nature 314(6012):642-644 (1985).
Hopwood et al., "Genes for Polyketide Secondary Metabolic Pathways in Microorganisms and Plants," Secondary Metabolites: Their Function and Evolution, Wiley Chichester (Ciba Foundation Symposium 171), pp. 88-112 (1992).
Hunaiti et al., "Source of Methylmalonyl-Coenzyme A for Erythromycin Synthesis: Methylmalonyl-Coenzyme A Mutase from Streptomyces erythreus," Antimicrobial Agents and Chemotherapy 25(2):173-178 (1984).
Kao et al., "Engineered Biosynthesis of a Complete Macrolactone in a Heterologous Host," Science 265:509-512 (1994).
Khosla et al., "Genetic Construction and Functional Analysis of Hybrid Polyketide Synthases Containing Heterologous Acyl Carrier Proteins," J. Bacteriol. 175(8):2197-2204 (1993).
Lanz et al., "The Role of Cysteines in Polyketide Synthase," J. of Biol. Chem. 266(15):9971-9976 (1991).
Leadlay et al., "The Erythromycin-Producing Polyketide Synthase," Biochem. Soc. Transactions 21:218-222 (1993).
MacNeil et al., "Complex Organization of the Streptomyces avermitilis Genes Encoding the Avermectin Polyketide Synthase," Gene 115:119-125 (1992).
Malpartida et al., "Molecular Cloning of the Whole Biosynthetic Pathway of a Streptomyces Antibiotic and its Expression in a Heterologous Host," Nature 309:462-464 (1984).
Malpartida et al., "Physical and Genetic Characterisation of the Gene Cluster for the Antibiotic Actinorhodin in Streptomyces coelicolor A3(2)," Mol. Gen. Genet. 205:66-73 (1986).
Marsden et al., "Stereospecific Acyl Transfers on the Erythromycin-Producing Polyketide Synthase," Science 263:378-380 (1994).
Roberts et al., "[.sup.3 H]Tetrahydrocerulenin, a Specific Reagent for Radio-Labeling Fatty Acid Synthases and Related Enzymes," FEBS Lett. 159(1,2):13-16 (1983).
Roberts et al., "Use of [.sup.3 H]Tetrahydrocerulenin to Assay Condensing Enzyme Activity in Streptomyces erythreus," Biochem. Soc. Transactions 12:642-643 (1984).
Shen et al., "Enzymatic Synthesis of a Bacterial Polyketide from Acetyl and Malonyl Coenzyme A," Science 262:1535-1540 (1993).
Sherman et al., "Structure and Deduced Function of the Granaticin-
Cane David E.
Kao Camilla
Khosla Chaitan
Luo Guanglin
Pieper Rembert
Brown University Research Foundation
MacMillan Keith D.
Stanford University
Wessendorf T. D.
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