Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1996-05-15
1997-07-22
Ketter, James
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4351736, 4352852, C12N 512
Patent
active
056503059
ABSTRACT:
A method and apparatus for electrofusing a cell pellet comprising a plurality of tiers of cells of two different cell types to be fused which results in the formation of hybrid cells with high efficiency and high viability. The method comprises subjecting the cell pellet, and a pulse medium in contact with the cell pellet, with multiple pulses of voltage. A method and apparatus for electrofusing a cell-vesicle pellet comprising a plurality of tiers of a cell type and of membrane vesicles, and wherein the membrane vesicles are loaded with an exogenous macromolecule to be delivered to the cell type during fusion, which results in the formation of fused cells containing the exogenous macromolecule with high efficiency and high viability. Alteratively, the membrane vesicles may contain at least one type of exogenous macromolecule inserted into its membrane. Fusion with a target cell results in the exogenous macromolecule being incorporated into the cell membrane of the target cell with high efficiency and high viability. The method comprises subjecting the cell-vesicle pellet, and a pulse medium in contact with the cell-vesicle pellet, with multiple pulses of voltage.
REFERENCES:
patent: 5007995 (1991-04-01), Takahashi et al.
Hensen Mary L.
Hui Sek Wen
Lin-Hong Li
Zhao Ya Li
Health Research , Inc.
Ketter James
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