Method to produce high levels of methioninase

Details Method to produce high levels of methioninase Method to produce high levels of methioninase

1996-05-03

1999-04-06

Lau, Kawai

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

4353201, 4352523, 43525233, 435 72, 435 732, 435 4, C12N 988, C12N 1563, C12N 121, C12Q 104

Patent

active

058917047

ABSTRACT:
The present invention discloses the cloning of a methioninase-encoding nucleic acid molecule from Pseudomonas putida and the construction of high-level expression modules containing the methioninase-encoding nucleic acid molecule. The invention further provides expression modules that use the T7 RNA polymerase promoter to express the isolated methioninase-encoding nucleic acid molecules. Expression modules employing the T7 promoter were found to produce unexpectedly high levels of methioninase. The present invention further provides purification methods to obtain highly pure, endotoxin free methioninase, chemically modified forms of methioninase, crystallized methioninase and lyophilized methioninase preparations. The present invention further provides therapeutical methods using the disclosed recombinant methioninase preparations.

REFERENCES:
patent: 4298590 (1981-11-01), Bogoch
patent: 4952496 (1990-08-01), Studier et al.
patent: 5028420 (1991-07-01), Masegi et al.
patent: 5487984 (1996-01-01), Allet et al.
patent: 5571510 (1996-11-01), Nobori et al.
Breillout, et al., "Methionine Dependency of Malignant Tumors: A Possible Approach for Therapy," J. of National Cancer Institute 82:1628-1632 and 1593-1660 (1990).
Chello, et al., "Dependence of 5-Methyltetrahydrofolate Utilization by L5178Y Murine Leukemia Cells In Vitro on the Presence of Hydroxycobalamin and Transcobalamin II," Cancer Res., 33:1898-1904 (1973).
Cohen, et al., "Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA," Proc. Natl. Acad. Sci. USA 69:2110-2114 (1972).
Dethy, et al., "Carbon-11-Methionine and Fluorine-18-FDG PET Study in Brian Hematoma" J. Nuclear Med. 35:1162-1166 (1994).
Eagle, "Nutrition Needs of Mammalian Cells in Tissue Culture" Science 122:501-504 (1955).
Eagle, "Amino Acid Metabolism in Mammalian Cell Cultures" Science 130:432-437 (1959).
Freeman, et al., "In Vivo-Like growth of Human Tumors In Vitro," Proc. Natl. Acad. Sci USA, 83:2694-2698 (1986).
Goseki, et al., "Antitumor Effect of Methionine-Depleting total Parenteral Nutrition with Doxorubicin Administration on Yoshida Sarcoma-Bearing rats" Cancer 69:1865-1872 (1992).
Graham, et al., "A New Techniques for the Assay of Infectivity of Human Adenoviruss 5 DNA," Virology 52:456-467 (1973).
Hernandez, et al., Los Liposomas, Entre Modelo de Membranas Biologicas y Transportadores de Principios Activos Cir. Farm. 293:49-60 (1987).
Hoffman, et al., "Reversible Growth arrest in Simian Virus 40-Transformed Human Fibroblasts," Proc. Natl. Acad. Sci. USA, 77:7306-7310 (1980).
Hoffman, et al., "A General Native-State Method for Determination of Proliferation Capacity of Human Normal and Tumor Tissues In Vitro," Proc. Natl. Acad. Sci. USA, 86:2013-2017 (1989).
Hoffman, et al., "Altered Methionine Metabolism, DNA Methylation and Oncogene Expression in Carcinogenesis," Biochem. Biophys. Acta 738:49-87 (1984).
Hoffman, et al., "Altered Methionine Metabolism and Transmethylation in Cancer" Anticancer Res. 5:1-30 (1985).
Huovinen, et al., "Carbon-11-methionine and PET in evaluation of treatment response of breast cancer," Br. J. Cancer 67:787-791 (1993).
Ito, et al., "Purification and Characterization of Methioninase from Pseudomonas putida" Biochemistry 79:1263-1272 (1976).
Kang, "Hyperhomocyst(e)inemia as a Risk Factor For Occlusive Vascular Disease," Annu. Rev. Nutr. 12:279-298 (1992).
Kreis, et al., "Biological Effects O Enzymatic Deprivation of L-Methionine in Cell Culture and Experimental Tumor," Cancer Research 33:1866-1869 (1973).
Kreis, et al., "Effect of Nutritional and Enzymatic Methionine Deprivation upon Human Normal and Malignant Cells in a Tissue Culture," Cancer Research 40:634-641 (1980).
Lapela, et al., "Imaging of Uterine Carcinoma by Carbon-11-Methionine and PET" J. Nucl. Med. 35:1618-23 (1994).
Leighton, "A Sponge Matrix Method for Tissue Culture" J. Nat'l Cancer Institute 12:545-561 (1951).
Leskinen-Kallio, et al., J. Nucl. Med. 33:691-695 (1992).
Leskinen-Kallio, et al., "Uptake of Carbon-11-Methionine and Fluorodeoxyglucose in Non-Hodgkin's Lymphoma: A PET Study," J. of Nuclear Medicine 32:1211-1218 (1991).
Lindholm, et al., "Comparison of Fluorine-18-Fluorodeoxyglucose and Carbon-11-Methionine in Head and Neck Cancer" J.Nucl. Med. 34:1711-1716 (1993).
Lishko, et al., Anticancer Research 13:1465-1468 (1993).
Lowry et al., "Protein Measurement with the Folin Phenol Reagent," J. Biol.Chem. 193:265 (1951).
Matteucci, et al., "Synthesis of Deoxyoligonucleotides on a Polymer Support," J. Am.Chem. Soc. 103:3185-3191 (1981).
McCully, "Vascular Pathology of Homocytseinemia: Implications for the Pathogenesis of Arteriosclerosis," Am.J. Pathology 56:111-128 (1969).
Mecham, et al., "The Metabolic Defect of Methionine Dependence Occurs Frequently in Human Tumor Cell Lines," Biochem. Biophys. Res Comm., 117:429-434 (1983).
Mineura, et al., "Innovative Approach in the Diagnosis of Gliomatosis Cerebri Using Carbon-11-L-Methionine Positron Emission Tomography," J. of Nuclear Med. 32:726-728 (1991).
Miyazawa, et al., "PET Imaging of Non-Small-Cell Lung Carcinoma with Carbon-11-Methionine: Relationship Between Radioactivity Uptake and Flow-Cytometric Parameters" J. Nucl. Med. 34:1886-1891 (1991).
Nakayama, et al., "Purification and Properties of L-Methionine Gamma-Lyase From Aeromonas SP," Agric. Biol. Chem. 48:2367-2369 (1984).
Nieto, et al., "Cloning vectors, derived from a naturally occuring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas," Gene 87:145-149 (1990).
Sambrook, et al., Molecular Cloning: A Laboratory Manual, Cold Sring Harbor Press, pp. A.1-A.4 (1989).
Sambrook, et al., Molecular Cloning: A Laboratory Manual, Cold Sring Harbor Press, pp. 129-1.30 (1989).
Sambrook, et al., Molecular Cloning: A Laboratory Manual, Cold Sring Harbor Press, pp. 1.74-1.84 (1989).
Sambrook, et al., Molecular Cloning: A Laboratory Manual, Cold Sring Harbor Press, pp. 18.47-18.59 (1989).
Selhub, et al., "Association Between Plasma Homocysteine Concentrations and Extracranial Carotid-Artery Stenosis," New England J. of Medicine 32:286-91 (1995).
Shields, et al., "Contribution of Labeled Carbon Dioxide to PET Imaging of Carbon-11-Labeled Compounds," J. Nucl. Med. 33:581-584 (1992).
Soda, "Microdetermination of D-Amino Acids and D-Amino Acid Oxidase Activity with 3-Methyl-2-benzothiazolone Hydrazone Hydrochloride," Analytical Biochemistry 25:228-235 (1968).
Southern, "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis," J. Mol. Biol. 98:503-517 (1975).
Southern et al., "Transformation of Mammalian Cells to Antibiotic Resistance with a Bacterial Gene Under Control of the SV40 Early Region Promoter" J. Mol. Anyl. Genet. 1:327-341 (1982).
Stampfer, et al., "Can Lowering Homocysteine Levels Reduce Cardiovascular Risk?" New England J. of Medicine 332:326-329 (1995).
Stampfer, et al., "A Prospective Study of Plasma Homocyst(e)ine and Risk of Myocardial Infarction in US Physicians" JAMA 268:877-881 (1992).
Stern, et al., "Enhanced In Vitro Selective Toxicity of Chemotherapeutic Agents for Human Cancer Cells Based on a Metabolic Defect," J. Nat't Cancer Institute 76:629-639 (1986).
Tanaka, et al., "Purification and Properties of Methioninase from Pseudomonas Ovalis," FEBS Letters, 66:307-311 (1976).
Tarcha, Polymers for Controlled Drug Delivery, CRC Press Boca Raton pp. 265-273 (1990).
Ueland, et al., "Plasma Homocysteine and Cardiovascular Disease" Atherosclerotic Cardiovascular Disease, Hemostasis, and Endothelial Function Marcel Dekler, New York, pp. 183-236 (1992).
Vescio, et al., "In vivo-Like Responses of Human Tumors Growing In three-Dimensional Gel-Supported Primary Culture," Proc. Natl. Acad. Sci. USA 84:5029-5033 (1987).
Wigler, et al., "DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cell," Proc. Natl. Acad. Sci. USA 76:1373-1376 (1979).
Young, et al., "Efficient isolation of genes by using antibody probes," Proc. Natl. Acad. Sci. USA 80:1194-1198 (1983).
Zar, Biostatistical Analysis, Pr

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