Method to produce granulocyte colony stimulating factor from imm

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Avian cell – per se

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435384, 530351, C12N 506, C12N 502, C07K 1452

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active

056912005

ABSTRACT:
Immortal avian T lymphocyte cell lines which produce and secrete immune lymphokines are disclosed. These cell lines may be produced from T cells recovered from fowl which have been hyperimmunized in vivo. The activated T cells are first exposed in vitro to a mitogen effective for secondary stimulation thereof, and then virally transformed to produce an immortal cell line. When cultured in vitro, the cell lines produce and secrete immune lymphokines which may be administered to fowl to increase their resistance to infections.

REFERENCES:
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Tellez, G.I., Kogut, M.H., Hargis, B.M., "Immunoprophylaxis of Salmonella enteritidis Infection by Lymphokines in Leghorn Chicks." Avian Diseases, 1993, vol. 37, pp. 1062-1070.
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Marmor, M.D., Benatar, T., Ratcliffe, M.J.H. "Retroviral Transformation In Vitro of Chicken T Cells Expressing Either .alpha./.beta. or .gamma./.delta. T Cell Receptors by Reticuloendotheliosis Virus Strain T," J. Exp. Med., The Rockefeller University Press, Mar. 1993, vol. 177, pp. 647-656.
Kogut, M.H., McGruder, E.D., Hargis, B.M., Corrier, D.E., DeLoach, J.R. "In Vivo Activation of Heterophil Function in Chickens Following Injection with Salmonella Enteritidis-Immune Lymphokines," Journal of Leukocyte Biology, Jan. 1995, vol. 57, pp. 56-62.

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