Drug – bio-affecting and body treating compositions – Extract – body fluid – or cellular material of undetermined... – Urogenital system
Patent
1991-04-26
1992-08-04
Robinson, Douglas W.
Drug, bio-affecting and body treating compositions
Extract, body fluid, or cellular material of undetermined...
Urogenital system
436 63, 436172, 435 2, A61K 3552
Patent
active
051357593
ABSTRACT:
Intact X and Y chromosome-bearing sperm populations of rabbits and swine were separated according to DNA content using a flow cytometer/cell sorter. Sperm viability was maintained by special staining techniques and by sorting and collecting the sperm in nutrient media. The sorted sperm were surgically inseminated into the uteri of rabbits or swine. Of the offspring born from does inseminated with the sorted population of X-bearing sperm, 94% were females. Of offspring born from does inseminated with sorted Y-bearing sperm from the same ejaculate, 81% were males.
REFERENCES:
patent: 4083957 (1978-04-01), Lang
patent: 4191749 (1980-03-01), Bryant
Culling, Handbook of Histopathological & Histochemical Techniques, Butterworth & Co., London, 1974, p. 192.
L. A. Johnson, "Flow Sorting of Intact X and Y Chromosome-Bearing Mammalian Spermatozoa," Cytometry, Suppl. 2, p. 66 Abstract (1988).
J. C. Conover and R. B. L. Gwatkin, "Pre-Loading of Mouse Oocytes with DNA-Specific Fluorochrome (Hoechst 33342) Permits Rapid Detection of Sperm-Oocyte Fusion," J. Reprod. Fert. 82: 681-690 (1988).
L. A. Johnson and R. N. Clarke, "Flow Sorting of X and Y Chromosome-Bearing Mammalian Sperm: Activation and Pronuclear Development of Sorted Bull, Boar, and Ram Sperm Microinjected into Hamster Oocytes," Gamete Res. 21: (1988).
L. A. Johnson et al., "Flow Cytometry of X and Y Chromosome-Bearing Sperm for DNA Using an Improved Preparation Method and Staining with Hoechst 33342," Gamete Res. 17: 203-212 (1987).
R. E. Hinkley et al., "Rapid Visual Detection of Sperm-Egg Fusion Using the DNA-Specific Fluorochrome Hoechst 33342," Dev. Biol. 118: 148-154 (1986).
L. A. Johnson and D. Pinkel, "Modification of a Laser-Based Flow Cytometer for High-Resolution DNA Analysis of Mammalian Spermatozoa," Cytometry 7: 268-273 (1986).
L. A. Johnson, "Gender Preselection in Farm Animals," Research for Tomorrow, 1986 Yearbook of Agriculture, pp. 73-77 (1986).
W. M. Grogan et al., "DNA Analysis and Sorting of Viable Mouse Testis Cells," J. Histochem. Cytochem. 29: 738-746 (1981).
M. R. Loken, "Separation of Viable T and B Lymphocytes Using a Cytochemical Stain, Hoechst 33342," J. Histochem. Cytochem. 28: 36-39 (1980).
Fado John D.
Ribando Curtis P.
Robinson Douglas W.
Silverstein M. Howard
The United States of America as represented by the Secretary of
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