Method to prepare nucleic acids from a biological sample using l

Details Method to prepare nucleic acids from a biological sample using l Method to prepare nucleic acids from a biological sample using l

1993-02-10

1995-01-31

Parr, Margaret

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2541, 536 2542, 435 6, 435 911, 435184, 435199, 435212, 435219, 436175, 935 19, C07H 2300, C12Q 168

Patent

active

053860241

ABSTRACT:
Method for making available a desired nucleic acid contained in a biological sample, comprising the steps of acidifying said biological sample to a pH at which endogenous nucleases capable of degrading the desired nucleic acid(s) are inactive, contacting said biological sample with an exogenous acid protease active at said pH, incubating said sample until endogenous nuclease activities are reduced to insignificant levels, and raising the pH of the biological sample to a pH sufficient to render the exogenous protease less active.

REFERENCES:
patent: 4900677 (1990-02-01), Hewitt
Naoumov et al. J. Clin. Pathol. (1988) 41:793-798.
Arrand, L. "Preparation of Nucleic Acid Probes" in Nucleic Acid Hybridisation a practical approach ed. Hames et al. pp. 17-20 (1985).
Calbiochem 1992 catalog p. 236 (1992).

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