Method to obtain intact, viable protoplasts from pollen grains

Details Method to obtain intact, viable protoplasts from pollen grains Method to obtain intact, viable protoplasts from pollen grains

1987-06-09

1992-12-08

Fox, David T.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524045, 43524054, C12N 502, C12N 500

Patent

active

051697769

ABSTRACT:
Intact, viable protoplast are obtained readily and in good quantity from within the cell walls of mature pollen grains. Pollen grains are contacted with an aqueous medium containing an osmolyte, gelatin, or a combination thereof in a concentration to expand the protoplast and force it out through the pore in the cell wall of the pollen grain, while maintaining the protoplast intact and viable.

REFERENCES:
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Evans et al., (1983) in Handbook of Plant Cell Culture, vol. 1, D. A. Evans et al., eds., Macmillan Publ. Co., N.Y., pp. 129 and 136.
Hawley (1981) The Condensed Chemical Dictionary, 10th Ed., Van Nostrand Reinhold Co., N.Y., p. 492.
Linskens, H. 1963, p. 143 In: Pollen physiology and fertilization, North-Holland Publishing Co., Amsterdam.
Bengochea et al., 1986, Plant protoplasts, p. 8, Chapman and Hall, New York.
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