Method to increase the trehalose content of organisms by transfo

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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4351723, 435193, 4352542, 43525421, 4353201, 435942, 536 232, 536 2374, 935 28, 935 29, 935 37, C12P 1918

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054222540

ABSTRACT:
Two nucleotide sequences encoding two different polypeptides found in yeast trehalose synthase have been isolated and cloned. The coding sequences can be inserted into suitable vectors and used to transform host cells. The transformed cells will produce increased amounts of trehalose compared to the untransformed wild types and have increased tolerance to a variety of stresses, in particular to decreased availability of water. The invention may be used to improve the stress tolerance of organisms, to increase the storage life of foodstuffs and to produce trehalose economically on an industrial scale in an organism (e.g, baker's yeast) that is a traditional and safe foodstuff.

REFERENCES:
patent: 4615974 (1986-10-01), Kingsman et al.
patent: 4794175 (1988-12-01), Nunberg et al.
patent: 4876197 (1989-10-01), Burke et al.
patent: 5312909 (1994-05-01), Driessen et al.
John Londesborough et al, "Trehalose-6-phosphate synthase/phosphatase complex from baker's yeast: purification of a proteolytically activated form", J. of Gen. Microbiology, vol. 137, pp. 323-330 (1991).
O. E. Vuorio et al, "Purification of Trehalose-6-phosphate Synthetase Phosphatase Complex from Baker's Yeast", Abstract, 20th FEBS Meeting, Aug. 1990, Budapest, Hungary. Abstract No. P-Tu 560.
Vania M. F. Paschoalin et al, "Identification of an ADPG-dependent trehalose synthase in Saccharomyces", Curr. Genet, vol. 16, pp. 81-87 (1989).
Anita D. Panek et al, "Metabolism and thermotolerance function of trehalose in Saccharomyces: a current perspective", J. of Biotech., vol. 14, pp. 229-238 (1990).
A. C. Panek et al, "Regulation of the trehalose-6-phosphate synthase in Saccharomyces", Curr. Genet, vol. 11, pp. 459-465 (1987).
Annick Vandercammen et al, "Characterization of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase of Saccharomyces cerevisiae", Eur. J. Biochem., vol. 182, pp. 613-620 (1989).
Paul V. Attfield, "Trehalose accumulates in Saccharomyces cerevisiae during exposure to agents that induce heat shock response", Elsevier Science, Publishers (Biomed. Div.), vol. 225, Nos. 1,2, pp. 259-263 (1987).
Marcelino Banuelos et al, "Saccharomyces carsbergensis fdp Mutant and Futile Cycling of Fructose 6-Phosphate Molecular and Cellular Biology", vol. 2, No. 8, pp. 921-929 (1982).
E. Cabib et al, "The Biosynthesis of Trehalose Phosphate", J. Biol Chem., vol. 231, pp. 259-275 (1957).
John H. Crowe et al "Stabilization od dry phospholipid bilayers and proteins by sugars", Biochem. J., vol. 242, pp. 1-10 (1987).
Tony D'Amore et al, "The involvement of trehalose in yeast stress tolerance", J. of Indust. Microbiol., vol. 7, 191-196 (1991).
G. M. Gadd et al, "The role of trehalose in dehydration resistance of Saccharomyces cerevisiae", FEMS Microbiology Letters vol. 48, pp. 249-254 (1987).
Pierre Gelinas et al "Effect of Growth Conditions and Trehalose Content on Cryotolerance of Bakers' Yeast in Frozen Doughs", Applied and Environmental Microbiol., vol. 55, No. 10, pp. 2453-2459 (1989).
Akihiro Hino et al, "Trehalose Levels and Survival Ratio of Freeze-Tolerant versus Freeze-Sensitive Yeasts", Applied and Environmental Microbiol., vol. 55, No. 5, pp. 1386-1391 (1990).
Thomas Hottiger et al, "Rapid changes of heat and desiccation tolerance correlated with changes of trehalose content in Saccharomyces cerevisiae cells subjected to temperature shifts", FEBS Letters, vol. 220, No. 1, pp. 113-115 (1987).
Thomas Hottiger et al, "Correlatin of trenalose content and heat resistance in yeast mutants altered in the RAS/adenylate cyclase pathway: is trehalose a thermoprotectant?", FEBS Letters, vol. 255, No. 2, pp. 431-434 (1989).
Rosario Lagunas et al, "Role of phosphate in the regulation of the Pasteur effect in Saccharomyces cerevisiae", Eur. J. Biochem, vol. 137, pp. 479-483 (1983).
U. K. Laemmli, "Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4", Nature, vol. 227, Aug. 15, 1970, pp. 680-685.
Gil Navon et al, "Phosphorus-31 Nuclear Magnetic Resonance Studies of Wild-Type and Glycolytic Pathway Mutants of Saccharomyces cerevisiae", Biochemistry, vol. 18, No. 21, pp. 4487-4499 (1979).
Maria Jose Neves et al, "Effects of heat shock on the level of trehalose and glycogen, and on the induction of thermotolerance in Neurospora crassa", FEBS Letters, vol. 283, No. 1, pp. 19-22 (1991).
Yuji Oda et al, "Selection of Yeasts for Breadmaking by the Frozen-Dough Method", Applied and Environmental Microbiology, vol. 56, No. 4, pp. 941-943 (1986).
G. R. Stewart, "Desiccation injury, anhydrobiosis and survival," in Plants Under Stress, Soc. Exper. Biol. Seminar, Series 39, Cambridge, UK (1989), pp. 115-130.
Patricia Tekamp-Olson et al, "The isolation, characterization and nucleotide sequence of the phosphoglucoisomerase gene of Saccharomyces cerevisiae", Gene, vol. 10, pp. 153-161 (1988).
K. W. van de Poll et al, "Characterization of a Regulatory Mutant of Frutose 1,6-Bisphosphatase in Saccharomyces carlsbergensis", MGG, pp. 61-66 (1977).
Andres Wiemken, "Trehalose in Yeast, Stress Protectant Rather Than Reserve Carbohydrate", Antonie van Leeuwenhoek, vol. 58, pp. 209-217 (1990).
Londesborough et al, Abstract, Gordon conference (on plant cold resistance) (1991).
Sankar Adhya et al, "Positive Control", J. Biolog. Chem., vol. 265, No. 19, pp. 10797-10800 (1990).
Emily C. Dale et al, "Gene transfer with subsequent removal of the selection gene from the host genome", Pro. Natl. Acad. Sci. USA, vol. 88, pp. 10558-10562, (1991).
David Pellman et al, "TATA-dependent and TATA-independent transcription at the HIS4 gene of yeast", Nature, vol. 348, pp. 82-85 (1990).
Gilbert Berben et al, "The YDp Plasmids: a Uniform Set of Vectors Bearing Versatile Gene Disruption Cassettes for Saccharomyces cerevisiae, Yeast", vol. 7, pp. 475-477 (1991).
Rodney J. Rothstein, "One-Step Gene Disruption in Yeast", Methods in Enzymology, vol. 101, pp. 203-243 (1983).
Patrick Russo et al, "Distinct cis-acting signals enhance 3' endpoint formation of CYC1 mRNA in the yeast Saccharomyces cerevisiae", The Embo J., vol. 10, No. 3, pp. 563-571 (1991).
Pirkko L. Suominen, "Characterization and Application of the Yeast Mell Gene", Dissertation (1988).
Lillie and Pringle, J. Bacteriology, vol. 143, pp. 1384-1394 (1980).
Bhown et al, "High-Sensitivity Sequence Determination . . . " An. Biochem. 103:184-190 (1980).
Giaever et al. "Biochemical and Genetic Characterization of Osmoregulatory Trehalose Synthesis . . . ", J. Bacteriol. 170:2841-2849, Jun. 1988.
Nelson et al, "A Conserved Gene Encoding the 57-kDa Subunit . . . " J. Biol. Chem. 264:1775-1778, Jan. 25, 1989.

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