Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1988-01-14
1991-06-25
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 697, 435 712, 4351723, 4352523, 4353201, 530380, 536 27, 935 27, 935 45, 935 51, 935 73, C12N 1567, C12N 1503, C12N 1512, C12P 2100
Patent
active
050266390
ABSTRACT:
The present invention provides a method to improve the expression of a poorly expressed recombinant protein, such as platelet factor 4, by constructing a gene fusion such that unfavorable secondary structure formed between the region of the messenger RNA specifying the recombinant protein and the region of the messenger RNA containing the ribosome binding site is disrupted. The gene fusion is constructed by inserting a DNA segment of sufficient length to disrupt unfavorable long-range folding interactions which render the ribosome binding site inaccesible to ribosomes thereby interfering with the translation of mRNA. The invention further provides for the expression of recombinant platelet factor 4 and provides recombinant DNA vectors and methods for the bacterial production and recovery of said platelet factor 4.
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Murphy Lisabeth Felix
Nippon Mining Company Limited
Schwartz Richard A.
Treptow Nancy
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